{"product_id":"96-well-anionic-protein-binding-plates-bhu16300003","title":"96-Well Anionic Protein-Binding Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBinding Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well Anionic Protein-Binding Plates bind negatively charged proteins. Proteins bound to the plate often keep their native conformation and their catalytic or binding activity, so a second molecule that needs the native structure can still recognize the bound protein.\u003c\/p\u003e\n\u003cp\u003eThe plates can be used for ELISA and for studies of protein-protein, protein-peptide, protein-DNA and protein-RNA interactions. The bound second molecule is detected by biochemical, biophysical or immunological methods.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003cp\u003eExample data: binding signal on a DNA-binding plate (left) and a protein-binding plate (right).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eAPP96B\u003c\/td\u003e\n\u003ctd\u003eAPP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinds\u003c\/th\u003e\n\u003ctd\u003eNegatively charged proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eAPP96B, APP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 200 µl of 0.5-1 mg\/ml protein in a low-salt buffer (10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) to each well and incubate at 4°C overnight, then rinse.\u003c\/li\u003e\n\u003cli\u003eBlock with 300 µl of blocker for 30 min: 1% BSA when the second molecule is a protein, or 1× Nucleic Acid Binding Blocker when it is a nucleic acid.\u003c\/li\u003e\n\u003cli\u003eAdd 200 µl of the second molecule in low-salt buffer and incubate for 1 hour, then rinse.\u003c\/li\u003e\n\u003cli\u003eDetect the bound second molecule, for example by enzyme activity, a fluorescent or radioactive label, or SYBR Green fluorescence (Ex 485 nm \/ Em 535 nm) for nucleic acids.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Protein-binding Plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe Nucleic Acid Binding Blocker used for nucleic acid partners is sold separately.\u003c\/li\u003e\n\u003cli\u003eThe second molecule concentration depends on its binding affinity; about 0.1 mg\/ml is typical for proteins, lower for antibodies, and micromolar for DNA oligos.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923319149,"sku":"APP96B","price":152.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923941741,"sku":"APP96T","price":152.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA-binding_plates_640_5e818fe8-74cd-46a5-a396-69911db1e79a.gif?v=1793049625","url":"https:\/\/www.ebiohippo.com\/products\/96-well-anionic-protein-binding-plates-bhu16300003","provider":"BioHippo","version":"1.0","type":"link"}