{"product_id":"96-well-dna-binding-plates-bhu16300002","title":"96-Well DNA-Binding Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eBinding Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well DNA-Binding Plates are polystyrene plates coated with positive charges that bind nucleic acids (DNA or RNA). They are used to study specific interactions between a bound nucleic acid and a second molecule, either another nucleic acid or a protein.\u003c\/p\u003e\n\u003cp\u003eThe nucleic acid is first bound to the plate through charge-charge interactions. After blocking, the plate is incubated with the second molecule, and any second molecule that binds to the immobilized nucleic acid is detected by biochemical or biophysical methods. The 96-well format can also be used to screen libraries of nucleic acids or proteins for binding to the immobilized nucleic acid.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003cp\u003eExample data: binding signal on a DNA-binding plate (left) and a protein-binding plate (right).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNBP96B\u003c\/td\u003e\n\u003ctd\u003eNBP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaterial\u003c\/th\u003e\n\u003ctd\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSurface\u003c\/th\u003e\n\u003ctd\u003ePositively charged; binds DNA and RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA Binding Capacity\u003c\/th\u003e\n\u003ctd\u003eAbout 1.1 ng\/mm² (measured with a 1,000-nt DNA oligo at 2 µg\/µl)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability of Bound DNA\u003c\/th\u003e\n\u003ctd\u003eStable below 200 mM salt, and with non-ionic detergents such as Triton X-100 and Brij-35 below their CMC; SDS interferes with DNA binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNBP96B, NBP96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 200 µl of 10 µg\/ml DNA in a low-salt buffer (10 mM buffer, 10 mM NaCl, 0.01% Tween-20, 1 mM EDTA) to each well and incubate at 4°C for 2 hours to overnight; overnight binding gives more complete binding.\u003c\/li\u003e\n\u003cli\u003eRinse, then block with 300 µl of blocker for 30 min: 1% BSA when the second molecule is a protein, or 1× Nucleic Acid Binding Blocker when it is a nucleic acid.\u003c\/li\u003e\n\u003cli\u003eAdd 200 µl of the second molecule in low-salt buffer and incubate for 1 hour, then rinse.\u003c\/li\u003e\n\u003cli\u003eDetect the bound second molecule, for example by enzyme activity, a fluorescent or radioactive label, or SYBR Green fluorescence (Ex 485 nm \/ Em 535 nm) for nucleic acids.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “96-well DNA–binding plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe Nucleic Acid Binding Blocker used for nucleic acid partners is sold separately.\u003c\/li\u003e\n\u003cli\u003eThe second molecule concentration depends on its binding affinity; about 0.1 mg\/ml is typical for proteins, lower for antibodies, and micromolar for DNA oligos.\u003c\/li\u003e\n\u003cli\u003eDetergents may affect protein function in the binding step.\u003c\/li\u003e\n\u003cli\u003eWhen the second molecule is a nucleic acid detected with SYBR Green, include wells with the bound DNA alone as background, because the dye also stains the immobilized DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923417453,"sku":"NBP96B","price":143.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923908973,"sku":"NBP96T","price":143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA-binding_plates_640.gif?v=1793049625","url":"https:\/\/www.ebiohippo.com\/products\/96-well-dna-binding-plates-bhu16300002","provider":"BioHippo","version":"1.0","type":"link"}