{"product_id":"96-well-super-high-binding-elisa-plates-bhu16300005","title":"96-Well Super High Binding ELISA Plates","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eELISA Plates\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe 96-Well Super High Binding ELISA Plates are designed to give strong ELISA signals. Strong protein binding to the plate surface increases ELISA sensitivity.\u003c\/p\u003e\n\u003cp\u003eIn the indirect format, the antigen is coated directly onto the plate, the plate is blocked, an antibody against the antigen is added, and the bound antibody is detected with an enzyme-linked secondary antibody. When two antibodies (A and B) against the antigen are available, antibody A can be coated first to capture the antigen, which is then detected with antibody B and an enzyme-linked antibody; this antibody pre-coating may further increase sensitivity.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 4 plates. Black plates give a low background for fluorescence detection. Transparent plates are for detection by light absorbance.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eBlack (fluorescence detection)\u003c\/th\u003e\n\u003cth\u003eTransparent (absorbance detection)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eELISA96B\u003c\/td\u003e\n\u003ctd\u003eELISA96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack\u003c\/td\u003e\n\u003ctd\u003eTransparent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003ctd\u003eLight absorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eContents\u003c\/th\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003ctd\u003e4 plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e96-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Color\u003c\/th\u003e\n\u003ctd\u003eBlack or transparent (two options; see Product Description)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e4 plates per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Coating Volume\u003c\/th\u003e\n\u003ctd\u003e100 µl per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Wash \/ Block Volume\u003c\/th\u003e\n\u003ctd\u003e360 µl per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eELISA96B, ELISA96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eCoat each well with 100 µl of antigen (2-fold dilution series in PBS with 0.02% sodium azide; a typical top concentration is 1 µg\/ml) and rotate at 4°C overnight.\u003c\/li\u003e\n\u003cli\u003eWash 4 times with 360 µl of PBS with 0.01% Tween-20, then block with 360 µl of PBS with 0.02% sodium azide and 3% fetal bovine serum for 2 hours at room temperature.\u003c\/li\u003e\n\u003cli\u003eWash, add 100 µl of primary antibody diluted in PBS with 0.02% sodium azide and 0.1% fetal bovine serum, and incubate with rotation for 1 hour at room temperature.\u003c\/li\u003e\n\u003cli\u003eWash, add 100 µl of the secondary antibody for 1 hour, wash again, add 200 µl of detection solution and read absorbance or fluorescence.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “96-well Super High Binding ELISA Plates” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFor antibody pre-coating, coat 100 µl of 1 µg\/ml antibody A at 4°C overnight or at 37°C for 2 hours, block, and then continue from the antigen step without a second blocking step.\u003c\/li\u003e\n\u003cli\u003eIn the antibody pre-coating format, antibody B should come from a different host species than antibody A, or be directly labeled, so that the enzyme-linked secondary antibody does not bind the capture antibody.\u003c\/li\u003e\n\u003cli\u003eSodium azide inhibits horseradish peroxidase (HRP); leave it out of buffers used with HRP-conjugated antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Black (fluorescence detection)","offer_id":67815923351917,"sku":"ELISA96B","price":160.0,"currency_code":"USD","in_stock":true},{"title":"Transparent (absorbance detection)","offer_id":67815923876205,"sku":"ELISA96T","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ELISA96_copy.jpg?v=1793049625","url":"https:\/\/www.ebiohippo.com\/products\/96-well-super-high-binding-elisa-plates-bhu16300005","provider":"BioHippo","version":"1.0","type":"link"}