{"product_id":"aav-cmv-null-aav-aav-null-bhv21600462","title":"AAV-CMV-Null AAV (AAV-Null)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAAV-CMV-Null AAV (AAV-Null) is an empty-cassette AAV control vector available across multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9) under the CMV promoter. It is the matched control for AAV transduction studies, allowing investigators to isolate payload-specific effects from background capsid- and dose-related effects.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapsid (serotype):\u003c\/strong\u003e available as AAV1, AAV2, AAV5, AAV6, AAV8, AAV9. Tropism summary: AAV1 — broad transduction with notable activity in skeletal muscle, central nervous system (neurons), and retina; AAV2 — efficient neuronal transduction with limited spread; widely used for in vitro work and for focal CNS injections; AAV5 — efficient transduction of airway epithelium and CNS neurons; widely used in the brain; AAV6 — effective transduction of skeletal and cardiac muscle, lung, and some hematopoietic populations; AAV8 — strong liver tropism upon systemic delivery; also active in muscle, CNS and retina; AAV9 — broad biodistribution after systemic delivery, including cardiac, skeletal muscle, and the central nervous system (the latter most efficient in neonatal animals).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter:\u003c\/strong\u003e CMV — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePayload:\u003c\/strong\u003e no transgene (empty vector) — cassette without a transgene insert; matched control vector for the same serotype\/promoter to account for AAV-related effects independent of the payload.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGenome backbone:\u003c\/strong\u003e Recombinant AAV (single-stranded unless explicitly noted as scAAV) flanked by AAV2 ITRs.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eEmpty-cassette (null) AAV vectors carry the same regulatory elements (ITRs, promoter, polyA) as the matched payload-encoding vector but lack a transgene insert. They are widely used as the matched control in AAV studies because they reproduce capsid biology, dose, and route of administration without producing a payload-specific protein product.\u003c\/p\u003e\u003cp\u003eBy comparing payload AAV to null AAV at the same titer, capsid, and promoter, investigators can isolate the contribution of the transgene from non-specific effects of capsid uptake, innate immunity, or vector dose.\u003c\/p\u003e\u003cp\u003eThe CMV promoter — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types — drives expression of the payload from the AAV cassette in this product. Promoter–capsid combinations together determine where and at what level the payload is expressed.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReporting standards for AAV studies increasingly emphasize matched-control comparisons to disentangle payload effects from capsid biology.\u003c\/li\u003e\n\u003cli\u003eAAV vector engineering — including capsid evolution, capsid shuffling, and rational design — continues to expand the spectrum of accessible tissues and cell types.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMatched control for AAV transduction studies.\u003c\/li\u003e\n\u003cli\u003eCapsid\/dose-comparison studies across capsids and routes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eUse this product within experimental designs that include matched controls (capsid, promoter, dose, route) and a transduction validation step before interpreting payload-specific phenotypes.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConfirm transduction efficiency in the target cell population before drawing payload-specific conclusions; reporter signal alone validates only that the vector reached and expressed in the cells.\u003c\/li\u003e\n\u003cli\u003eMatch AAV dose, capsid, promoter, and route across all conditions when comparing payload to control; differences in any of these confound payload-specific interpretation.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles of AAV stocks — aliquot upon first thaw.\u003c\/li\u003e\n\u003cli\u003eAAV biology, including tropism, can differ between species, strains, ages, and routes — confirm in your specific system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Mingozzi F, High KA. Immune responses to AAV vectors: overcoming barriers to successful gene therapy. Blood. 2013. https:\/\/ashpublications.org\/blood\/article\/122\/1\/23\/31819\n- Wang D, Tai PWL, Gao G. Adeno-associated virus vector as a platform for gene therapy delivery. Nat Rev Drug Discov. 2019. https:\/\/www.nature.com\/articles\/s41573-019-0012-9\n- Schultz BR, Chamberlain JS. Recombinant adeno-associated virus transduction and integration. Mol Ther. 2008. https:\/\/www.cell.com\/molecular-therapy-family\/molecular-therapy\/fulltext\/S1525-0016(16)33063-5\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"AAV1 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53325150585197,"sku":"7025","price":495.0,"currency_code":"USD","in_stock":true},{"title":"AAV2 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53325153108333,"sku":"7026","price":495.0,"currency_code":"USD","in_stock":true},{"title":"AAV5 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53325172801901,"sku":"7028","price":495.0,"currency_code":"USD","in_stock":true},{"title":"AAV6 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53325174309229,"sku":"7029","price":495.0,"currency_code":"USD","in_stock":true},{"title":"AAV8 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53286541623661,"sku":"7077","price":495.0,"currency_code":"USD","in_stock":true},{"title":"AAV9 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53325175652717,"sku":"7030","price":495.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/aav-cmv-null-aav-aav-null-bhv21600462","provider":"BioHippo","version":"1.0","type":"link"}