{"product_id":"aav-gfp-aav-serotype-5-aav-aav5-gfp-bhv21600449","title":"AAV-GFP (AAV serotype 5) AAV (AAV5-GFP)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAAV-GFP (AAV serotype 5) AAV (AAV5-GFP) is an AAV vector packaged in AAV5 under the CMV promoter that delivers \u003cstrong\u003eeGFP\u003c\/strong\u003e to mammalian cells. Researchers commonly use this vector for reporter assay; in vivo gene delivery; cell labeling.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapsid (serotype):\u003c\/strong\u003e AAV5. efficient transduction of airway epithelium and CNS neurons; widely used in the brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter:\u003c\/strong\u003e CMV — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePayload:\u003c\/strong\u003e eGFP — enhanced green fluorescent protein; standard fluorescent reporter.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGenome backbone:\u003c\/strong\u003e Recombinant AAV (single-stranded unless explicitly noted as scAAV) flanked by AAV2 ITRs.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eeGFP is an enhanced variant of green fluorescent protein originally cloned from \u003cem\u003eAequorea victoria\u003c\/em\u003e. It folds autonomously in mammalian cells and produces a chromophore by cyclization of an internal Ser-Tyr-Gly tripeptide. eGFP excitation and emission peaks (~488 nm excitation \/ ~507 nm emission) are well-matched to standard fluorescence microscopy and flow cytometry hardware.\u003c\/p\u003e\u003cp\u003eBecause eGFP signal is proportional (within limits) to expression level, eGFP-expressing AAVs are commonly used as reporters of transduction efficiency, promoter activity, and targeting fidelity.\u003c\/p\u003e\u003cp\u003eThe CMV promoter — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types — drives expression of the payload from the AAV cassette in this product. Promoter–capsid combinations together determine where and at what level the payload is expressed.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReporter AAVs continue to be standard tools for benchmarking new capsid variants and engineered serotypes (e.g., directed evolution capsids, MyoAAV-class capsids, AAV.PHP-family capsids in mice).\u003c\/li\u003e\n\u003cli\u003eSingle-cell readouts (e.g., scRNA-seq, FACS) increasingly use reporter AAVs to define transduced populations within heterogeneous tissue.\u003c\/li\u003e\n\u003cli\u003eAAV vector engineering — including capsid evolution, capsid shuffling, and rational design — continues to expand the spectrum of accessible tissues and cell types.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eTracking transduction efficiency across cell types or tissue regions.\u003c\/li\u003e\n\u003cli\u003eBenchmarking new capsids or promoters in vitro and in vivo.\u003c\/li\u003e\n\u003cli\u003eActing as a labeling vector in dual-color experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eUse this product within experimental designs that include matched controls (capsid, promoter, dose, route) and a transduction validation step before interpreting payload-specific phenotypes.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConfirm transduction efficiency in the target cell population before drawing payload-specific conclusions; reporter signal alone validates only that the vector reached and expressed in the cells.\u003c\/li\u003e\n\u003cli\u003eMatch AAV dose, capsid, promoter, and route across all conditions when comparing payload to control; differences in any of these confound payload-specific interpretation.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles of AAV stocks — aliquot upon first thaw.\u003c\/li\u003e\n\u003cli\u003eAAV biology, including tropism, can differ between species, strains, ages, and routes — confirm in your specific system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998. https:\/\/www.annualreviews.org\/doi\/10.1146\/annurev.biochem.67.1.509\n- Cormack BP, Valdivia RH, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996. https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/0378111995007343\n- Schultz BR, Chamberlain JS. Recombinant adeno-associated virus transduction and integration. Mol Ther. 2008. https:\/\/www.cell.com\/molecular-therapy-family\/molecular-therapy\/fulltext\/S1525-0016(16)33063-5\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"AAV5 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53286504497517,"sku":"7006","price":495.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/aav-gfp-aav-serotype-5-aav-aav5-gfp-bhv21600449","provider":"BioHippo","version":"1.0","type":"link"}