{"product_id":"aav-rfp-icre-aav2-aav-aav2-rfp-icre-bhv21600486","title":"AAV-RFP-iCre (AAV2) AAV (AAV2-RFP-iCre)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAAV-RFP-iCre (AAV2) AAV (AAV2-RFP-iCre) is an AAV vector packaged in AAV2 under the CMV promoter that delivers \u003cstrong\u003ecodon-improved Cre (iCre)\u003c\/strong\u003e to mammalian cells. Researchers commonly use this vector for conditional gene knockout\/activation in floxed mouse lines; lineage tracing.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapsid (serotype):\u003c\/strong\u003e AAV2. efficient neuronal transduction with limited spread; widely used for in vitro work and for focal CNS injections.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter:\u003c\/strong\u003e CMV — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePayload:\u003c\/strong\u003e codon-improved Cre (iCre) — mammalian codon-optimized Cre recombinase variant with reduced CpG content and improved expression in mammalian cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGenome backbone:\u003c\/strong\u003e Recombinant AAV (single-stranded unless explicitly noted as scAAV) flanked by AAV2 ITRs.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eiCre is a mammalian codon-improved variant of Cre recombinase from bacteriophage P1. The codon optimization reduces silencing in mammalian cells and increases expression relative to unmodified Cre. Cre catalyzes site-specific recombination between two 34-bp loxP sites, enabling deletion (loxP-loxP), inversion (DIO\/FLEX), or activation (LSL stop cassette) of floxed gene cassettes in conditional mouse models.\u003c\/p\u003e\u003cp\u003eAAV-delivered iCre is a widely used tool for delivering Cre activity to a specific cell population through capsid tropism and promoter selectivity, particularly when a tissue-specific Cre driver line is unavailable.\u003c\/p\u003e\u003cp\u003eThe CMV promoter — human cytomegalovirus immediate-early promoter; strong, broadly active in most mammalian cell types — drives expression of the payload from the AAV cassette in this product. Promoter–capsid combinations together determine where and at what level the payload is expressed.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCre-AAV is widely used to deliver Cre activity to brain regions or cell types without dedicated driver lines, expanding the conditional genetics toolkit.\u003c\/li\u003e\n\u003cli\u003eCombinations of intersectional approaches (Cre + Flp, or CreERT2 + tamoxifen pulses) allow finer spatial and temporal restriction of recombination.\u003c\/li\u003e\n\u003cli\u003eAAV vector engineering — including capsid evolution, capsid shuffling, and rational design — continues to expand the spectrum of accessible tissues and cell types.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConditional gene knockout in floxed mouse alleles.\u003c\/li\u003e\n\u003cli\u003eActivation of Cre-dependent reporter lines (e.g., Ai9\/Ai14, Rosa-LSL-LacZ).\u003c\/li\u003e\n\u003cli\u003eLineage tracing when delivered to a defined cell population.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eUse this product within experimental designs that include matched controls (capsid, promoter, dose, route) and a transduction validation step before interpreting payload-specific phenotypes.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConfirm transduction efficiency in the target cell population before drawing payload-specific conclusions; reporter signal alone validates only that the vector reached and expressed in the cells.\u003c\/li\u003e\n\u003cli\u003eMatch AAV dose, capsid, promoter, and route across all conditions when comparing payload to control; differences in any of these confound payload-specific interpretation.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles of AAV stocks — aliquot upon first thaw.\u003c\/li\u003e\n\u003cli\u003eAAV biology, including tropism, can differ between species, strains, ages, and routes — confirm in your specific system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Sauer B, Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. PNAS. 1988. https:\/\/www.pnas.org\/doi\/10.1073\/pnas.85.14.5166\n- Shimshek DR, et al. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis. 2002. https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.10023\n- Atasoy D, Aponte Y, Su HH, Sternson SM. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. J Neurosci. 2008. https:\/\/www.jneurosci.org\/content\/28\/28\/7025\n- Schultz BR, Chamberlain JS. Recombinant adeno-associated virus transduction and integration. Mol Ther. 2008. https:\/\/www.cell.com\/molecular-therapy-family\/molecular-therapy\/fulltext\/S1525-0016(16)33063-5\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"AAV2 \/ 1x10^13 GC\/ml \/ 20 µL","offer_id":53286504857965,"sku":"7081","price":495.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/aav-rfp-icre-aav2-aav-aav2-rfp-icre-bhv21600486","provider":"BioHippo","version":"1.0","type":"link"}