{"product_id":"abcb1-human-and-abcg2-human-stable-overexpression-mdck-cell-line-negative-clone-31-bhc10923445","title":"ABCB1(Human) and ABCG2 (Human) Stable Overexpression MDCK Cell Line - Clone #31","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe ABCB1(Human) and ABCG2 (Human) Stable Overexpression MDCK Cell Line is a specialized tool designed for advanced pharmacological and biochemical research. This cell line is derived from MDCK (Madin-Darby Canine Kidney) cells, which have been genetically engineered to stably overexpress human ABCB1 and ABCG2 genes.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e ABCB1(Human) and ABCG2 (Human) Stable Overexpression MDCK Cell Line - Clone #31 is supplied as a tumor cell line derived from Dog kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks    (G299)    or coating your preferred vessels with Applied Cell Extracellular Matrix    (G422).  Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.    \n*Do not heat-inactivate\n\n1.5 µg\/ml Puromycin (G264) for selection.\n Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cancer biology, phenotype comparison, and response profiling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eABCB1 and ABCG2 are crucial members of the ATP-binding cassette (ABC) transporter family, known for their role in multidrug resistance (MDR) and drug efflux mechanisms. T9814 is suitable for studying the pharmacokinetics and drug interaction dynamics of potential therapeutics, particularly in understanding how drugs are transported across cellular membranes and their efficacy in overcoming multidrug resistance. This cell line is instrumental for researchers focusing on cancer therapy, drug development, and membrane transport biology, providing invaluable insights into drug resistance mechanisms and the development of more effective treatment strategies. Donor\/background information provided for this product: Female, Adult, Normal, Cocker Spaniel.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks    (G299)    or coating your preferred vessels with Applied Cell Extracellular Matrix    (G422).  Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.    \n*Do not heat-inactivate\n\n1.5 µg\/ml Puromycin (G264) for selection.\n Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 24h\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e \u003cp\u003e1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/p\u003e\n\u003cp\u003e2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003c\/p\u003e\n\u003cp\u003e3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003c\/p\u003e\n\u003cp\u003e4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003c\/p\u003e\n\u003cp\u003e5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.\u003c\/p\u003e\n\u003cp\u003e6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period.\"\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"font-size:0.85em;color:#555555;border-top:1px solid #e0e9e9;padding-top:10px;margin-top:20px;\"\u003e\u003cstrong\u003eMaterial Citation:\u003c\/strong\u003e If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T9814\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180557427053,"sku":"T9814","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DUY8o2yeFBSirgWGIo2sFsEiXPEbYn7PEWXC8Z0G.png?v=1774957896","url":"https:\/\/www.ebiohippo.com\/products\/abcb1-human-and-abcg2-human-stable-overexpression-mdck-cell-line-negative-clone-31-bhc10923445","provider":"BioHippo","version":"1.0","type":"link"}