{"product_id":"abce1-antibody-atp-binding-cassette-sub-family-e-member-1-bha17135817","title":"ABCE1 Antibody \/ ATP-binding cassette sub-family E member 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eABCE1 Antibody \/ ATP-binding cassette sub-family E member 1 is a anti-ABCE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ABCE1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eABCE1 antibody detects ATP-binding cassette sub-family E member 1, an essential translation factor involved in ribosome recycling and antiviral defense. Encoded by the ABCE1 gene on chromosome 4q31.21, this highly conserved protein belongs to the ATP-binding cassette (ABC) transporter superfamily but is unique in lacking transmembrane domains. Instead, ABCE1 functions in the cytoplasm as a ribonucleoprotein-associated ATPase that promotes ribosome dissociation after translation termination, enabling efficient ribosome recycling and translation reinitiation.\u003cbr\u003e\u003cbr\u003eStructurally, ABCE1 is a 599-amino-acid protein of approximately 68 kilodaltons composed of two nucleotide-binding domains (NBDs) and an N-terminal region containing iron-sulfur clusters critical for its activity. These clusters facilitate conformational changes required for ribosome disassembly. ABCE1 interacts with release factors (eRF1 and eRF3) and ribosomal subunits, coupling ATP hydrolysis to subunit separation. The protein is ubiquitously expressed and localized in the cytoplasm, with enrichment near polysomes.\u003cbr\u003e\u003cbr\u003eThe ABCE1 antibody is widely used in molecular biology, virology, and translational control research to study ribosome dynamics, translation termination, and antiviral mechanisms. Western blot analysis detects a 68 kilodalton band corresponding to ABCE1, while immunofluorescence shows diffuse cytoplasmic distribution associated with ribosomal clusters. This antibody enables exploration of how ATPase-driven ribosome recycling maintains translation efficiency and proteostasis.\u003cbr\u003e\u003cbr\u003eFunctionally, ABCE1 acts as a �ribosome recycling factor,� splitting 80S ribosomes into 40S and 60S subunits after peptide release. It also inhibits RNase L activation, thereby modulating innate antiviral responses. In viruses such as HIV-1, ABCE1 facilitates Gag assembly, linking translation control to viral replication. The ABCE1 gene is essential for cell viability, and its disruption leads to impaired translation and cell death. The ABCE1 antibody provides a powerful tool for studying the intersection of translation regulation, ribosome turnover, and antiviral defense.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ABCE1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ABCE1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ABCE1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ABCE1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ABCE1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305994605,"sku":"FY12915","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_df9e5802-63e6-4e38-a770-448dc9fd85aa.jpg?v=1782237057","url":"https:\/\/www.ebiohippo.com\/products\/abce1-antibody-atp-binding-cassette-sub-family-e-member-1-bha17135817","provider":"BioHippo","version":"1.0","type":"link"}