{"product_id":"abcg5-antibody-atp-binding-cassette-sub-family-g-member-5-bha17135788","title":"ABCG5 Antibody \/ ATP-binding cassette sub-family G member 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eABCG5 Antibody \/ ATP-binding cassette sub-family G member 5 is a anti-ABCG5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ABCG5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eABCG5 antibody detects ATP-binding cassette sub-family G member 5, a sterol transporter that regulates cholesterol and plant sterol efflux. Encoded by the ABCG5 gene on chromosome 2p21, this half-transporter functions as a heterodimer with ABCG8 to form an ATP-dependent transporter localized to the apical membranes of enterocytes and hepatocytes. The ABCG5\/ABCG8 complex limits intestinal sterol absorption and promotes biliary cholesterol excretion, maintaining systemic lipid balance.\u003cbr\u003e\u003cbr\u003eStructurally, ABCG5 is a 651-amino-acid integral membrane glycoprotein of approximately 70 kilodaltons that contains one nucleotide-binding domain (NBD) and one transmembrane domain (TMD). It requires heterodimerization with ABCG8 for stability and activity, forming a functional exporter that utilizes ATP hydrolysis to transport sterols across lipid bilayers. ABCG5 is highly expressed in liver, small intestine, and gallbladder epithelium, where it contributes to cholesterol homeostasis and xenosterol elimination.\u003cbr\u003e\u003cbr\u003eThe ABCG5 antibody is widely used in lipid metabolism, hepatology, and cardiovascular research to study sterol transport, bile secretion, and cholesterol regulation. Western blot analysis detects a 70 kilodalton band corresponding to ABCG5, while immunofluorescence reveals localization to apical plasma membranes of enterocytes and canalicular membranes of hepatocytes. This antibody enables detailed investigation of sterol transport mechanisms and lipid trafficking defects underlying metabolic disorders.\u003cbr\u003e\u003cbr\u003eLoss-of-function mutations in ABCG5 cause sitosterolemia, a rare lipid disorder characterized by excessive absorption and accumulation of dietary plant sterols and premature atherosclerosis. Conversely, increased ABCG5 expression contributes to enhanced sterol efflux and protection against hypercholesterolemia. The ABCG5\/ABCG8 complex is also regulated by nuclear receptors such as liver X receptor (LXR) and farnesoid X receptor (FXR), integrating lipid metabolism with transcriptional control. The ABCG5 antibody provides a high-quality reagent for monitoring transporter expression, assessing sterol efflux activity, and exploring therapeutic regulation of cholesterol metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ABCG5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ABCG5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ABCG5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ABCG5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ABCG5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305175405,"sku":"FY12886","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8bc5b1e4-be4c-4b6c-9081-27de04b7ae1b.jpg?v=1782237063","url":"https:\/\/www.ebiohippo.com\/products\/abcg5-antibody-atp-binding-cassette-sub-family-g-member-5-bha17135788","provider":"BioHippo","version":"1.0","type":"link"}