{"product_id":"abr-antibody-active-breakpoint-cluster-region-related-protein-bha17135529","title":"ABR Antibody \/ Active breakpoint cluster region-related protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eABR Antibody \/ Active breakpoint cluster region-related protein is a anti-ABR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ABR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eABR antibody detects Active breakpoint cluster region-related protein, a GTPase-activating protein that regulates the Rho family of small GTPases including Rac and Cdc42. ABR modulates cytoskeletal organization, cell migration, and neuronal development by controlling GTPase signaling cycles. The ABR antibody is widely used in neuroscience, immunology, and cancer biology to study cytoskeletal regulation, signaling, and morphogenesis.\u003cbr\u003e\u003cbr\u003eABR is encoded by the ABR gene located on human chromosome 17p13.3. The protein is approximately 871 amino acids long and contains a GTPase-activating (GAP) domain and a guanine nucleotide exchange factor (GEF)-like domain, giving it dual regulatory capacity. ABR localizes to the cytoplasm and plasma membrane, where it modulates actin cytoskeleton remodeling and cell polarity.\u003cbr\u003e\u003cbr\u003eThe ABR antibody detects a 98 kilodalton protein by western blot and shows perinuclear and membrane-associated staining under immunofluorescence. ABR acts as a negative regulator of Rac and Cdc42 by promoting their conversion from active GTP-bound to inactive GDP-bound states. This modulation is essential for neuronal growth cone collapse, immune cell migration, and epithelial polarity.\u003cbr\u003e\u003cbr\u003eIn neurons, ABR contributes to axon guidance and dendritic spine development, while in immune cells it regulates phagocytosis and chemotaxis. Loss of ABR activity leads to hyperactivation of Rho GTPases, resulting in abnormal morphology and impaired signal transduction. In cancers, aberrant ABR expression alters cell adhesion, invasion, and metastatic behavior.\u003cbr\u003e\u003cbr\u003eBeyond cytoskeletal control, ABR interacts with signaling molecules involved in inflammation and oxidative stress. It participates in cellular defense mechanisms by modulating NADPH oxidase activity and reactive oxygen species production. These diverse functions establish ABR as a central hub linking signaling to structural adaptation.\u003cbr\u003e\u003cbr\u003eAs a bidirectional regulator of GTPase signaling, ABR provides key insights into cell dynamics and tissue morphogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ABR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ABR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ABR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ABR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ABR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294755181,"sku":"FY12626","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b748e416-639d-4927-8bd8-027fc62e614e.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/abr-antibody-active-breakpoint-cluster-region-related-protein-bha17135529","provider":"BioHippo","version":"1.0","type":"link"}