{"product_id":"acan-crispr-knockout-rcs-cell-line-rat-bhc10900959","title":"Acan CRISPR Knockout RCS Cell Line (Rat)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eAcan\u003c\/em\u003e (Aggrecan) has been disrupted in the RCS (Rat Chondrosarcoma, Swarm strain) cell line background. RCS cells are a well-established model for chondrocyte biology and cartilage extracellular matrix (ECM) studies; the Acan knockout removes the primary cartilage proteoglycan to enable loss-of-function analysis. Cells grow as adherent and suspension cultures and are supplied frozen (BSL-II).\u003c\/p\u003e\n\n\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eAcan\u003c\/em\u003e (Aggrecan; rat gene symbol)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated disruption of the \u003cem\u003eAcan\u003c\/em\u003e locus with stable integration confirmed by selection\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e RCS — Rat Chondrosarcoma (Swarm rat chondrosarcoma origin); adherent and suspension growth; bone\/cartilage tissue model\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eGene Background\u003c\/h2\u003e\n\u003cp\u003eAggrecan (ACAN\/Acan) is the most abundant proteoglycan in articular cartilage and is essential for the compressive biomechanical properties of the tissue. Structurally, aggrecan consists of a core protein decorated with glycosaminoglycan chains (chondroitin sulfate and keratan sulfate), which associate with hyaluronan and link proteins to form large aggregating complexes in the pericellular and territorial matrix. Aggrecan expression is regulated developmentally and is considered a marker of chondrocyte differentiation. Loss or degradation of aggrecan by aggrecanases (ADAMTS family enzymes) is associated with cartilage degradation in osteoarthritis and other musculoskeletal diseases. The RCS cell line is a commonly used in vitro model that retains chondrocytic characteristics and produces cartilage matrix components.\u003c\/p\u003e\n\n\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\n\u003cdl\u003e\n  \u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent and suspension\u003c\/dd\u003e\n  \u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eRat (\u003cem\u003eR. norvegicus\u003c\/em\u003e)\u003c\/dd\u003e\n  \u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eBone (Swarm rat chondrosarcoma)\u003c\/dd\u003e\n  \u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n  \u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003cp\u003eKnockout is confirmed by the presence of frameshift-inducing INDELs, verified by Sanger Sequencing.\u003c\/p\u003e\n\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCartilage ECM biology:\u003c\/strong\u003e Investigate the role of aggrecan in proteoglycan network assembly, hyaluronan binding, and matrix hydration in the RCS chondrosarcoma model.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAggrecanase target validation:\u003c\/strong\u003e Use the Acan KO line as a negative control to distinguish aggrecan-specific from off-target effects of ADAMTS inhibitors.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eChondrocyte differentiation:\u003c\/strong\u003e Compare chondrocytic marker expression (Col2a1, Sox9, Col10a1) between Acan KO and parental RCS to assess aggrecan's role in differentiation signaling.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eGlycosaminoglycan analysis:\u003c\/strong\u003e Quantify sulfated glycosaminoglycan secretion (by DMMB assay or Alcian blue staining) in KO vs. parental to establish aggrecan's contribution to total GAG output.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eImportant Notes\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eMixed growth mode:\u003c\/strong\u003e RCS cells can grow in both adherent and suspension states; culture vessel coating may influence growth behavior. Monitor cell attachment and morphology post-thaw.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor\/source:\u003c\/strong\u003e Parental RCS cells originate from the Swarm rat chondrosarcoma model (\u003cem\u003eR. norvegicus\u003c\/em\u003e).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eViability warranty:\u003c\/strong\u003e Cell viability upon thaw is warranted for 30 days following shipment when handled according to abm's cell handling guidelines.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c!-- Sources (internal):\n- ACAN gene entry | UniProt (P16112 rat) | https:\/\/www.uniprot.org\/uniprot\/P16112\n- Acan gene record | NCBI Gene ID: 25065 | https:\/\/www.ncbi.nlm.nih.gov\/gene\/25065\n- Watanabe H et al. (1994) Mouse cartilage matrix deficiency (cmd) caused by a 7 bp deletion in the aggrecan gene. Nat Genet | https:\/\/doi.org\/10.1038\/ng0794-261\n- Tortorella MD et al. (1999) Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins. Science | https:\/\/doi.org\/10.1126\/science.284.5420.1664\n- Bhosale AM \u0026 Richardson JB (2008) Articular cartilage: structure, injuries and review of management. Br Med Bull | https:\/\/doi.org\/10.1093\/bmb\/ldn025\n--\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180707864941,"sku":"T3000","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YgULxQgeWHc5tVrbDbqauzrouumfMJ1XBkFkbQ7K.png?v=1774960837","url":"https:\/\/www.ebiohippo.com\/products\/acan-crispr-knockout-rcs-cell-line-rat-bhc10900959","provider":"BioHippo","version":"1.0","type":"link"}