{"product_id":"ace-antibody-angiotensin-i-converting-enzyme-bha17135960","title":"ACE Antibody \/ Angiotensin I Converting Enzyme","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eACE Antibody \/ Angiotensin I Converting Enzyme is a anti-ACE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ACE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eACE antibody detects Angiotensin I converting enzyme, a zinc-dependent peptidase that plays a pivotal role in blood pressure regulation and electrolyte balance. The UniProt recommended name is Angiotensin I converting enzyme (ACE). This membrane-bound enzyme catalyzes the conversion of angiotensin I to the potent vasoconstrictor angiotensin II and degrades bradykinin, thereby modulating vascular tone and fluid homeostasis.\u003cbr\u003e\u003cbr\u003eFunctionally, ACE antibody identifies a 1,306-amino-acid type I membrane glycoprotein composed of two homologous catalytic domains (N- and C-domains) and a short cytoplasmic tail. ACE is expressed in endothelial cells, epithelial cells, and kidney proximal tubules, where it acts as a key enzyme of the renin-angiotensin system (RAS). By generating angiotensin II, ACE promotes vasoconstriction, sodium retention, and aldosterone secretion, contributing to blood pressure regulation.\u003cbr\u003e\u003cbr\u003eThe ACE gene is located on chromosome 17q23.3 and encodes multiple isoforms, including somatic ACE and testis-specific ACE (tACE). Somatic ACE is primarily found on the luminal surface of endothelial cells in lungs, heart, and kidneys, whereas tACE functions in sperm maturation and fertility. ACE expression is regulated by inflammatory cytokines, hormonal signaling, and mechanical stress on vascular endothelium.\u003cbr\u003e\u003cbr\u003eClinically, dysregulation of ACE activity is linked to hypertension, heart failure, diabetic nephropathy, and atherosclerosis. ACE inhibitors, which block enzyme activity, are among the most effective therapeutics for cardiovascular disease management. Beyond cardiovascular roles, ACE contributes to immune function by degrading inflammatory peptides and influencing hematopoietic stem cell mobilization. Elevated ACE levels are also diagnostic for sarcoidosis and other granulomatous disorders.\u003cbr\u003e\u003cbr\u003eACE antibody is widely used in cardiovascular, renal, and molecular physiology research. It is suitable for immunohistochemistry, western blotting, and flow cytometry to detect ACE expression and distribution in tissues. This antibody supports studies of vascular biology, RAS signaling, and peptide metabolism. In clinical and translational settings, ACE serves as a biomarker for endothelial activation and inflammatory vascular disease.\u003cbr\u003e\u003cbr\u003eStructurally, ACE consists of two catalytic domains containing zinc-binding motifs (HEMGH sequence) that coordinate enzymatic hydrolysis of peptide bonds. Its extracellular domain is heavily glycosylated, conferring stability and resistance to proteolysis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ACE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ACE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ACE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ACE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ACE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310942573,"sku":"FY13058","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e15e68c7-2ffc-4cb4-a3af-363c48b67cb1.jpg?v=1782237080","url":"https:\/\/www.ebiohippo.com\/products\/ace-antibody-angiotensin-i-converting-enzyme-bha17135960","provider":"BioHippo","version":"1.0","type":"link"}