{"product_id":"acvr2b-orf-cdna-lentivirus-bhv19400119","title":"ACVR2B ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eACVR2B encodes activin receptor type 2B, a transmembrane serine\/threonine kinase receptor of the TGF-β superfamily. It serves as a type II receptor for activins, myostatin, and related ligands; upon ligand binding it recruits and activates type I receptors, which phosphorylate SMAD transcription factors to control gene expression. ACVR2B signaling regulates skeletal muscle growth, embryonic patterning, and tissue homeostasis. Because myostatin signaling through ACVR2B restrains muscle mass, the receptor is a focus of research into muscle-wasting conditions, and it is also studied in development and cancer biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ACVR2B ORF cDNA Lentivirus delivers the human ACVR2B open reading frame for stable overexpression in mammalian cells. Expression of the ORF, fused to a C-terminal epitope tag, is driven by a CMV or EF1α promoter, with a fluorescent reporter (GFP, RFP, or BFP) and optional blasticidin or puromycin selection marker separated by self-cleaving peptide sequences for independent translation. The lentiviral vectors are optimized for high expression and reliable genome integration, supporting establishment of long-term stable cell lines. ORF accuracy is confirmed by sequencing and protein expression validated by transient transfection.\u003c\/p\u003e\n\u003cp\u003eSupplied as high-titer, third-generation, VSV-G-pseudotyped particles, it efficiently transduces primary and thawed cells for gain-of-function studies of activin and TGF-β signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of ACVR2B (NCBI Accession: NM_001106) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (BFP, GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-ACVR2B-V5 \/ BFP\/Blasticidin \/ 2x10^6","offer_id":53251618767213,"sku":"LCV-0027-1","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-ACVR2B-V5 \/ GFP\/Puromycin \/ 2x10^6","offer_id":53251634266477,"sku":"LCV-0027-2","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-ACVR2B-V5 \/ RFP\/Blasticidin \/ 2x10^6","offer_id":53251634299245,"sku":"LCV-0027-3","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/acvr2b-orf-cdna-lentivirus-bhv19400119","provider":"BioHippo","version":"1.0","type":"link"}