{"product_id":"adh6-antibody-alcohol-dehydrogenase-6-bha17135650","title":"ADH6 Antibody \/ Alcohol dehydrogenase 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eADH6 Antibody \/ Alcohol dehydrogenase 6 is a anti-ADH6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ADH6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eADH6 antibody detects Alcohol dehydrogenase class V (also known as ADH6), a zinc-dependent enzyme that catalyzes the oxidation of alcohols to aldehydes as part of ethanol and retinoid metabolism. Encoded by the ADH6 gene on chromosome 4q23, this enzyme belongs to the alcohol dehydrogenase family of oxidoreductases that use NAD+ as a cofactor. ADH6 is mainly expressed in liver tissue and contributes to the metabolism of short-chain alcohols and other substrates, complementing the activities of ADH1 and ADH4 isoenzymes.\u003cbr\u003e\u003cbr\u003eStructurally, ADH6 is a homodimer of approximately 40 kilodalton subunits, each containing a zinc-binding catalytic domain. The enzyme exhibits broad substrate specificity, oxidizing both ethanol and retinol intermediates. While its catalytic efficiency toward ethanol is lower than ADH1A and ADH1B, ADH6 plays a significant role in the oxidation of aromatic and aliphatic alcohols, making it important for detoxification and vitamin A metabolism. Genetic variations in ADH6 influence alcohol metabolism rates and susceptibility to alcohol-related diseases.\u003cbr\u003e\u003cbr\u003eThe ADH6 antibody is used in enzymology, metabolism, and toxicology research to study alcohol oxidation and hepatic metabolism. Western blot analysis identifies a 40 kilodalton band corresponding to ADH6, while immunohistochemistry shows strong cytoplasmic staining in hepatocytes. This antibody supports investigation into liver enzyme expression profiles, ethanol metabolism, and the biochemical pathways linking alcohol consumption to liver injury.\u003cbr\u003e\u003cbr\u003eBeyond hepatic metabolism, ADH6 participates in retinoid oxidation, converting retinol to retinal, a precursor of retinoic acid involved in gene regulation and differentiation. Dysregulation of ADH6 activity may impact vitamin A homeostasis and oxidative stress responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ADH6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ADH6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ADH6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ADH6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ADH6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298818413,"sku":"FY12747","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_27094e87-9904-4d24-9b30-eda98e9d9600.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/adh6-antibody-alcohol-dehydrogenase-6-bha17135650","provider":"BioHippo","version":"1.0","type":"link"}