{"product_id":"aer61-antibody-eogt-bha17135441","title":"AER61 Antibody \/ EOGT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAER61 Antibody \/ EOGT is a anti-AER61 Rabbit antibody Recombinant Rabbit Monoclonal clone 30E02 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AER61\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30E02, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEOGT antibody detects EGF domain specific O linked N acetylglucosamine transferase, an enzyme encoded by the EOGT gene. EOGT catalyzes the addition of O GlcNAc moieties to EGF like domains of secreted and membrane proteins, modifying signaling molecules and receptors. This post translational modification regulates ligand receptor interactions and signaling pathways critical for development and homeostasis.\u003cbr\u003e\u003cbr\u003eEOGT antibody is widely used in studies of glycosylation, signaling, and developmental biology. By detecting EOGT, researchers can explore how O GlcNAc modification influences Notch signaling, growth factor binding, and tissue morphogenesis. Mutations in EOGT are associated with Adams Oliver syndrome, a developmental disorder characterized by limb and scalp defects, underscoring its biological importance.\u003cbr\u003e\u003cbr\u003e In western blot assays, EOGT antibody detects protein bands corresponding to the enzyme in tissue and cell extracts. Immunohistochemistry maps expression in developing tissues, while immunofluorescence reveals subcellular localization consistent with endoplasmic reticulum and Golgi compartments. These methods support detailed analysis of glycosyltransferase biology.\u003cbr\u003e\u003cbr\u003eEOGT mediated O GlcNAc modification contributes to signaling regulation in vascular and neural development. Dysregulation of this pathway has implications in congenital disorders, cancer, and immune regulation. By applying EOGT antibody, scientists can investigate how defects in protein glycosylation contribute to human disease and identify therapeutic strategies that target glycosylation pathways.\u003cbr\u003e\u003cbr\u003eEOGT antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AER61 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AER61 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AER61 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AER61 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AER61+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047293280621,"sku":"FY12538","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_51a31d61-f049-40ec-a494-523dae2f2392.jpg?v=1772019305","url":"https:\/\/www.ebiohippo.com\/products\/aer61-antibody-eogt-bha17135441","provider":"BioHippo","version":"1.0","type":"link"}