{"product_id":"affinipure-mouse-anti-rabbit-igg-h-and-l-secondary-antibody-tritc-conjugated-bha21012530","title":"AffiniPure Mouse Anti-Rabbit IgG (H+L) secondary antibody, TRITC Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAffiniPure Mouse Anti-Rabbit IgG (H+L) secondary antibody, TRITC Conjugated is a Cy3 conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Rabbit IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e E. coli (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Rabbit IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Cy3 provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e The antibody was purified from antiserum by immunoaffinity chromatography..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Concentrated, Liquid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This CY3 conjugated Protein A, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eContents (provided):\u003c\/strong\u003e 1 mg\/ml of CY3 conjugated specific antibody; 0.01M PBS, 50% glycerol..\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence\/ICC:\u003c\/strong\u003e enables cellular localization and colocalization studies with multi-channel detection when spectra are compatible.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631267693,"sku":"BA1033-0.5ml","price":150.0,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072635691373,"sku":"BA1033-1ml","price":280.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/affinipure-mouse-anti-rabbit-igg-h-and-l-secondary-antibody-tritc-conjugated-bha21012530","provider":"BioHippo","version":"1.0","type":"link"}