{"product_id":"akr1c2-antibody-biotin-conjugated-bha10500235","title":"AKR1C2 Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-AKR1C2 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. Designed to detect AKR1C2 in Human, supporting researchers in signal transduction contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Aldo-keto reductase family 1 member C2 protein (1-323AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A\/G compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin conjugate:\u003c\/strong\u003e Signal amplification via streptavidin\/avidin-coupled reporters. Block endogenous biotin in liver, kidney, or biotin-supplemented samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAKR1C2\u003c\/strong\u003e (also known as 2-dihydrobenzene-1, 2-diol dehydrogenase, 3 alpha HSD3, 3 alpha hydroxysteroid dehydrogenase type III, 3-alpha-HSD3, AK1C2_HUMAN, AKR1C pseudo, AKR1C2, Aldo keto reductase family 1 member C2, Aldo-keto reductase family 1 member C2, BABP, Bile acid binding protein, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRD, DD, DD-2, DD\/BABP, DD2, DDH 2, DDH2, Dihydrodiol dehydrogenase 2, Dihydrodiol dehydrogenase\/bile acid binding protein, Dihydrodiol dehydrogenase\/bile acid-binding protein, FLJ53800, HAKRD, HBAB, MCDR 2, MCDR2, OTTHUMP00000044759, Pseudo chlordecone reductase, SRXY8, Trans 1 2 dihydrobenzene 1 2 diol dehydrogenase, Trans-1, Type II dihydrodiol dehydrogenase, Type III 3 alpha hydroxysteroid dehydrogenase, Type III 3-alpha-hydroxysteroid dehydrogenase) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of AKR1C2 biology relevant to signal transduction.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA quantifies soluble\/secreted target in biological fluids or cell culture supernatants.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Include IgG from Rabbit at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity\/isoforms:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53238394978669,"sku":"CSB-PA001543HD01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53238500884845,"sku":"CSB-PA001543HD01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA001543HA01HU-WB_e0a17445-889e-4277-b308-e2d870fa0ca2.jpg?v=1782237418","url":"https:\/\/www.ebiohippo.com\/products\/akr1c2-antibody-biotin-conjugated-bha10500235","provider":"BioHippo","version":"1.0","type":"link"}