{"product_id":"akt1-2-3-antibody-bha17135597","title":"AKT1\/2\/3 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAKT1\/2\/3 Antibody is a anti-AKT1\/2\/3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31A24 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AKT1\/2\/3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31A24, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAKT1\/2\/3 antibody detects the three isoforms of AKT, also known as protein kinase B, encoded by the AKT1, AKT2, and AKT3 genes. These serine threonine kinases are central regulators of cell survival, growth, metabolism, and angiogenesis. AKT proteins are activated downstream of phosphoinositide 3 kinase signaling in response to growth factors and insulin. Each isoform has overlapping and unique functions, ensuring precise control of cellular physiology.\u003cbr\u003e\u003cbr\u003eAKT1\/2\/3 antibody is widely applied in cancer biology, metabolism research, and neuroscience. AKT1 regulates cell survival and growth, AKT2 controls glucose metabolism, and AKT3 influences brain development and function. Dysregulation of AKT signaling is a hallmark of many cancers, diabetes, and neurological diseases. By detecting all three isoforms, researchers can evaluate global AKT activity and isoform specific contributions.\u003cbr\u003e\u003cbr\u003e Western blot assays detect AKT isoforms in diverse tissues, immunohistochemistry maps AKT distribution in tumors and metabolic organs, and immunofluorescence reveals cytoplasmic and membrane localization upon activation. ELISA provides quantitative measurement of AKT levels in clinical and experimental samples. These applications make AKT1\/2\/3 antibody a versatile reagent for broad pathway analysis.\u003cbr\u003e\u003cbr\u003eAKT kinases regulate multiple downstream targets including mTOR, FOXO transcription factors, and GSK3. They promote protein synthesis, inhibit apoptosis, and modulate metabolism. Aberrant AKT signaling drives oncogenesis, drug resistance, and metabolic disorders. By applying AKT1\/2\/3 antibody, scientists can investigate how PI3K AKT pathways integrate signals from growth factors, nutrients, and stress.\u003cbr\u003e\u003cbr\u003eTherapeutically, AKT inhibitors are under development for cancer and metabolic disease. Measuring AKT expression with antibody based assays provides biomarkers for drug development and patient stratification. AKT1\/2\/3 antibody therefore supports translational research linking kinase biology to clinical intervention.\u003cbr\u003e\u003cbr\u003eAKT1\/2\/3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AKT1\/2\/3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AKT1\/2\/3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AKT1\/2\/3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AKT1\/2\/3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AKT1\/2\/3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047296655725,"sku":"FY12694","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_19c108f8-a14b-449a-8ddb-82c8c71bc0d5.jpg?v=1772019332","url":"https:\/\/www.ebiohippo.com\/products\/akt1-2-3-antibody-bha17135597","provider":"BioHippo","version":"1.0","type":"link"}