{"product_id":"all-in-one-5x-rt-mastermix-with-gdna-removal-bht10900006","title":"All-In-One 5X RT MasterMix with gDNA Removal","description":"\u003cp\u003eThe \u003cstrong\u003eAll-In-One 5X RT MasterMix with gDNA Removal\u003c\/strong\u003e offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, \u003cstrong\u003eall in a single tube\u003c\/strong\u003e. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis.\u003c\/p\u003e\n\u003cp\u003eThe optimized MasterMix includes \u003cstrong\u003eabm\u003c\/strong\u003e’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo(dT)s and Random Primers. The OneScript® Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for enhanced cDNA yield and integrity. By eliminating the need for multiple separate reagents, this all-in-one mix offers a straightforward, reliable, and reproducible workflow that consistently delivers high-quality cDNA, even from challenging or low-quality RNA samples—ideal for a wide range of downstream molecular biology applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEasy one-step setup reduces pipetting and sample handling\u003c\/li\u003e\n\u003cli\u003eRemoves contaminating gDNA with temperature-sensitive DNase\u003c\/li\u003e\n\u003cli\u003eHigh cDNA yields from difficult samples with OneScript\u003csup\u003e®\u003c\/sup\u003e Hot Reverse Transcriptase (Cat. No. G593)\u003c\/li\u003e\n\u003cli\u003eInhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G138)\u003c\/li\u003e\n\u003cli\u003eAlso includes dNTPs, Oligo (dT)s, and random primers\u003c\/li\u003e\n\u003cli\u003eIncluded in \u003cstrong\u003eabm\u003c\/strong\u003e's \u003ca\u003ePCR Buffet Program\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"margin: 30px 0;\"\u003e\u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin-bottom: 20px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eProduct Component\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003eAll-In-One 5X RT MasterMix\u003c\/td\u003e\n\u003ctd\u003e100 rxn (400 µl)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-Free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\u003ctd\u003e1.0 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003ctable style=\"border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\" border=\"0\" cellpadding=\"10\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"vertical-align: middle;\"\u003e\n\u003ctd style=\"text-align: left; vertical-align: middle;\" width=\"15%\"\u003e\u003cimg src=\"\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\" alt=\"ISO 13485:2016 MDSAP Certified\" width=\"85%\"\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\"\u003e\n\u003cp\u003e\u003cstrong\u003eISO 13485:2016 MDSAP Certified\u003c\/strong\u003e\u003cbr\u003eOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 rxn","offer_id":53311769969005,"sku":"G592","price":209.0,"currency_code":"USD","in_stock":true},{"title":"10 rxns","offer_id":53311771017581,"sku":"Sample-G592","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/all-in-one-5x-rt-mastermix_product-image.jpg?v=1778555686","url":"https:\/\/www.ebiohippo.com\/products\/all-in-one-5x-rt-mastermix-with-gdna-removal-bht10900006","provider":"BioHippo","version":"1.0","type":"link"}