{"product_id":"alox5ap-antibody-arachidonate-5-lipoxygenase-activating-protein-bha17136170","title":"ALOX5AP Antibody \/ Arachidonate 5-lipoxygenase-activating protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eALOX5AP Antibody \/ Arachidonate 5-lipoxygenase-activating protein is a anti-ALOX5AP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: ER, nuclear membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ALOX5AP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eALOX5AP antibody detects Arachidonate 5-lipoxygenase-activating protein, a membrane-bound lipid-binding protein that plays an essential role in leukotriene biosynthesis. The UniProt recommended name is Arachidonate 5-lipoxygenase-activating protein (ALOX5AP). This protein facilitates the interaction between arachidonic acid and the enzyme 5-lipoxygenase (ALOX5), enabling the production of leukotrienes that mediate inflammation and immune responses.\u003cbr\u003e\u003cbr\u003eFunctionally, ALOX5AP antibody identifies a 161-amino-acid integral membrane protein located primarily in the nuclear envelope and endoplasmic reticulum. ALOX5AP functions as a scaffold that transfers arachidonic acid to ALOX5, catalyzing the first steps of leukotriene synthesis�conversion of arachidonic acid to leukotriene A4. This process produces potent signaling molecules that regulate vascular permeability, smooth muscle contraction, and leukocyte recruitment during inflammation.\u003cbr\u003e\u003cbr\u003eThe ALOX5AP gene is located on chromosome 13q12.3 and is highly expressed in leukocytes, macrophages, and endothelial cells. Expression increases in response to pro-inflammatory stimuli such as cytokines, lipopolysaccharides, and oxidative stress, linking ALOX5AP to innate immunity and inflammatory signaling networks.\u003cbr\u003e\u003cbr\u003ePathologically, ALOX5AP has been strongly associated with chronic inflammatory diseases including asthma, atherosclerosis, and stroke. Genetic polymorphisms in ALOX5AP are linked to increased cardiovascular risk and altered leukotriene production. Overactivation contributes to endothelial dysfunction and plaque instability. Research using ALOX5AP antibody supports studies in lipid mediator biosynthesis, immune cell signaling, and vascular inflammation.\u003cbr\u003e\u003cbr\u003eALOX5AP antibody is validated for western blotting, immunohistochemistry, and ELISA to detect lipid metabolism-associated proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Arachidonate 5-lipoxygenase-activating protein is an integral membrane protein containing four transmembrane helices that form a hydrophobic pocket for arachidonic acid binding and transfer. It interacts directly with ALOX5 at the nuclear membrane, coordinating substrate channeling during leukotriene biosynthesis. This antibody enables precise examination of ALOX5AP's role in lipid mediator production and inflammatory regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ALOX5AP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ALOX5AP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ALOX5AP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ALOX5AP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ALOX5AP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320510829,"sku":"FY13268","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ac28ce1e-1693-498c-a8ff-469e4058dea9.jpg?v=1782237075","url":"https:\/\/www.ebiohippo.com\/products\/alox5ap-antibody-arachidonate-5-lipoxygenase-activating-protein-bha17136170","provider":"BioHippo","version":"1.0","type":"link"}