{"product_id":"alpha-actin-antibody-acta2-smooth-muscle-actin-bha17136371","title":"Alpha Actin Antibody \/ ACTA2 \/ Smooth Muscle Actin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAlpha Actin Antibody \/ ACTA2 \/ Smooth Muscle Actin is a anti-ACTA2 Rabbit antibody Recombinant Rabbit Monoclonal clone CAD-1 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ACTA2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone CAD-1, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAlpha Actin antibody is a key reagent for studying cytoskeletal dynamics, smooth muscle physiology, and tissue remodeling. The encoded protein, ACTA2 (actin alpha 2), is a smooth muscle specific isoform of actin that contributes to contraction, structure, and motility of vascular and visceral smooth muscle cells. As a fundamental component of the cytoskeleton, ACTA2 polymerizes into filaments that provide mechanical strength and coordinate intracellular signaling.\u003cbr\u003e\u003cbr\u003eAlpha actin is highly expressed in the smooth muscle layers of blood vessels, the respiratory tract, the gastrointestinal system, and reproductive organs. It also serves as a well established marker for myofibroblasts, which emerge during wound healing and fibrotic disease. Upregulation of ACTA2 in fibroblasts indicates their transition into contractile myofibroblasts, a process central to tissue repair and pathologic fibrosis. Detection with Alpha Actin antibody allows researchers to track these changes in both normal physiology and disease progression.\u003cbr\u003e\u003cbr\u003eIn cardiovascular research, ACTA2 is examined as a marker of vascular smooth muscle cells and their response to injury or remodeling. Phenotypic switching of smooth muscle cells, from a contractile to a synthetic state, is associated with vascular disease such as atherosclerosis. In liver, kidney, and lung fibrosis, ACTA2 positive myofibroblasts accumulate and contribute to excessive extracellular matrix deposition. Because of this, the Alpha Actin antibody is routinely applied in histological studies of fibrotic disorders.\u003cbr\u003e\u003cbr\u003eAt the molecular level, ACTA2 shares high sequence similarity with other actin isoforms but contains unique features that confer smooth muscle specific function. It interacts with actin binding proteins to regulate filament turnover, contractility, and cell migration. The strong conservation of actin family proteins ensures that Alpha Actin antibody provides reliable detection across species in research models.\u003cbr\u003e\u003cbr\u003eThe Alpha Actin antibody is widely used in immunohistochemistry, immunofluorescence, western blotting, and flow cytometry. These applications enable localization and quantification of ACTA2 in tissue samples and cultured cells. For researchers focused on vascular biology, tissue fibrosis, or cytoskeletal organization, the Alpha Actin antibody is a specific and dependable detection tool.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ACTA2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ACTA2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ACTA2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ACTA2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ACTA2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047327031661,"sku":"RQ8953","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d9b15dc8-fd09-4f8d-8711-f48166db73bc.jpg?v=1772019452","url":"https:\/\/www.ebiohippo.com\/products\/alpha-actin-antibody-acta2-smooth-muscle-actin-bha17136371","provider":"BioHippo","version":"1.0","type":"link"}