{"product_id":"alpha-synuclein-luciferase-bioluminescent-stable-h4-cell-line-h4-s1s2-bhc10923016","title":"α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis H4 neuroglioma cell line stably expresses bioluminescent-protein complementation pairs of alpha-synuclein (α-synuclein) and luciferase (synLuc1\/synLuc2) under tetracycline regulatable expression (tet-off). α-synuclein is implicated in Lewy body related disorders like Parkinsons, dementia, Lewy bodies, and multiple system atrophy. Therefore, this cell line is suitable for studies investigating the pathogenic role of α-synuclein in such diseases.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2) is supplied as a tumor cell line derived from Human brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like with large cytoplasm; elongated and spindly when too confluent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS(Regular*) + 200ug\/ml Hygromycin B (G265) + 200ug\/ml G418 (G262) + 1 ug\/ml tetracycline* + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Expression of the construct is tetracycline regulated - TET-OFF***Cell growth and proliferation is better when seeded at higher densities; do not seed too sparsely** *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Luciferase reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eComplementation of α-synuclein with luciferase allows for in vitro imaging to investigate protein misfolding and aggregation. Control cell line:Cat. T3427 - Human Wild-Type Alpha-Synuclein H4 Cell Line Cat. T3428 - Full-length Gaussia Luciferase Expressing H4 Stable Cell Line (HgLuc) Complete list of cell lines from this panel:Cat. T3429 - α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2) Cat. T3430 - α-Synuclein-Venus Fluorescent Stable H4 Cell Line (H4 V1S\/SV2)Cat. T3441 - Full-length Venus Expressing H4 Stable Cell Line (HgLuc) Cat. T3458 - Mutant Alpha-Synuclein (Ala53Thr) Stable H4 Cell LineCat. T3459 - Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line Donor\/background information provided for this product: Male, White, 37, Neuorglioma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS(Regular*) + 200ug\/ml Hygromycin B (G265) + 200ug\/ml G418 (G262) + 1 ug\/ml tetracycline* + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Expression of the construct is tetracycline regulated - TET-OFF***Cell growth and proliferation is better when seeded at higher densities; do not seed too sparsely** *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 22,000 - 32,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180548809069,"sku":"T3429","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SXW5E9DSIFuPRIiO7GGiUjaOCkiSEVvVM6WOG104.png?v=1774957877","url":"https:\/\/www.ebiohippo.com\/products\/alpha-synuclein-luciferase-bioluminescent-stable-h4-cell-line-h4-s1s2-bhc10923016","provider":"BioHippo","version":"1.0","type":"link"}