{"product_id":"alpha-tubulin-antibody-tuba1c-bha17135847","title":"Alpha Tubulin Antibody \/ TUBA1C","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAlpha Tubulin Antibody \/ TUBA1C is a anti-TUBA1C Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TUBA1C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAlpha Tubulin antibody detects Alpha tubulin, a fundamental structural component of microtubules, which are integral parts of the cytoskeleton responsible for cell shape, intracellular transport, and mitotic spindle formation. The UniProt recommended name is Tubulin alpha chain, encompassing multiple isoforms encoded by genes such as TUBA1A, TUBA1B, and TUBA1C. Tubulins polymerize to form dynamic microtubule filaments that continually undergo assembly and disassembly, enabling cellular motility, division, and intracellular trafficking.\u003cbr\u003e\u003cbr\u003eFunctionally, Alpha Tubulin antibody recognizes a conserved 50 kDa cytoskeletal protein that pairs with beta-tubulin to form heterodimers, the basic building blocks of microtubules. These structures are critical for maintaining cell polarity, providing tracks for motor proteins like kinesin and dynein, and ensuring accurate chromosome segregation during mitosis. Tubulin polymerization is regulated by GTP binding and hydrolysis, and is sensitive to factors such as temperature, post-translational modifications, and microtubule-associated proteins (MAPs). The equilibrium between polymerized microtubules and soluble tubulin dimers governs many cellular functions, including vesicle transport and organelle positioning.\u003cbr\u003e\u003cbr\u003eAlpha tubulin undergoes various modifications such as acetylation, tyrosination, detyrosination, and polyglutamylation, which influence microtubule stability and motor protein interactions. Acetylated alpha tubulin is often used as a marker for stable microtubules, particularly in cilia and flagella. The Alpha Tubulin antibody is therefore widely applied as a loading control in western blotting, an internal cytoskeletal marker in immunofluorescence, and a reference protein for quantifying microtubule organization.\u003cbr\u003e\u003cbr\u003eThe TUBA gene family encodes several alpha-tubulin isoforms with tissue-specific expression patterns. TUBA1A is the predominant neuronal isoform and plays a major role in axon guidance and brain development. Mutations in TUBA1A have been associated with lissencephaly, cortical dysplasia, and microcephaly. Non-neuronal alpha-tubulin isoforms are equally essential for mitotic spindle integrity and intracellular transport in proliferating cells. Disruption of alpha-tubulin function, through chemical inhibitors such as colchicine or vinblastine, arrests cells in metaphase by depolymerizing microtubules.\u003cbr\u003e\u003cbr\u003eAlpha Tubulin antibody provides a robust marker for assessing cytoskeletal architecture, cellular morphology, and proliferation. It is frequently used in combination with antibodies against beta tubulin, gamma tubulin, or actin to visualize cytoskeletal dynamics. In research involving neurobiology, alpha tubulin serves as a key indicator of axonal growth and neuronal polarity. In cancer studies, changes in tubulin expression and post-translational modification patterns reflect cytoskeletal reorganization during transformation, invasion, and drug resistance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TUBA1C - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TUBA1C - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TUBA1C - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TUBA1C - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TUBA1C+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306944877,"sku":"FY12945","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_45692ef9-100e-48ae-9915-8a73e511c6cc.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/alpha-tubulin-antibody-tuba1c-bha17135847","provider":"BioHippo","version":"1.0","type":"link"}