{"product_id":"ampullary-neuroendocrine-carcinoma-cell-line-nec1452-bhc10902103","title":"Ampullary Neuroendocrine Carcinoma Cell Line (NEC1452)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eNEC1452 originates from an ampullary neuroendocrine carcinoma, developed to enhance the understanding and treatment of aggressive neuroendocrine cancers. This cell line was meticulously created from patient tumor samples, followed by successful propagation in immunocompromised mice, leading to consistent PDX models.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Ampullary Neuroendocrine Carcinoma Cell Line (NEC1452) is supplied as a tumor cell line derived from Human colon.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, clumps\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS(Regular*) + 1% L-Glutamine (G275) + 10µg\/ml insulin + 10mM nicotinamide + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eUnlike NEC913, NEC1452 does not express somatostatin receptor 2 (SSTR2) but shares similar high-grade characteristics with a Ki-67 index of 80-90%. The cell line maintains key neuroendocrine markers such as chromogranin A and synaptophysin, and exhibits critical genetic alterations in TP53 and RB1, mirroring the molecular profile of advanced neuroendocrine carcinomas. NEC1452 cells thrive in culture, both in suspension and as three-dimensional spheroids, providing a versatile platform for in vitro and in vivo studies. This model is particularly suited for exploring therapeutic avenues in cases where SSTR2-targeted treatments are not viable, thereby broadening the scope of neuroendocrine carcinoma research and therapeutic innovation.Additional cell lines from this panel: Cat. T8174 - Rectal Neuroendocrine Carcinoma Cell Line (NEC913) Donor\/background information provided for this product: Female, 38, Rectal NEC.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS(Regular*) + 1% L-Glutamine (G275) + 10µg\/ml insulin + 10mM nicotinamide + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 120\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180540682605,"sku":"T8175","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nqbHSaEEg4xH0SykSdCZGS55r0XdhEcjIsbtOu4U.png?v=1774957857","url":"https:\/\/www.ebiohippo.com\/products\/ampullary-neuroendocrine-carcinoma-cell-line-nec1452-bhc10902103","provider":"BioHippo","version":"1.0","type":"link"}