{"product_id":"ana-12-bhb21300008","title":"ANA-12","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e N-[2-[(2-oxoazepan-3-yl)carbamoyl]phenyl]-1-benzothiophene-2-carboxamide.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 219766-25-3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C22H21N3O3S.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 100-200 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TrkB receptor\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e ANA-12 is a selective TrkB antagonist, shown to bind directly and selectively to TrkB, preventing activation by BDNF and inhibiting processes downstream of TrkB, in a 2-site mode of action (IC50 values are 45.6 nM and 41.1 μM for the high and low affinity sites, respectively), without altering TrkA and TrkC functions. In adult mice ANA-12 decreases TrkB activity in the brain without affecting neuronal survival, and reduces anxiety and depression related behaviors1.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eANA-12 is a low molecular weight ligand that acts as a selective tropomyosin receptor kinase B (TrkB) antagonist. The compound shows direct binding to TrkB receptor and prevents its activation by BDNF with high potency, without altering TrkA and TrkC functions. The compound is considered to be a valuable tool for studying the BDNF-TrkB signaling pathway1,2.A study indicated that administration of ANA-12 has successfully reduced anxiety and depression related behaviors tested in mice1.TrkB receptor and BDNF are key mediators in the pathophysiology of several mood disorders, including drug addiction, anxiety and depression. They play an important role in the regulation of synaptic transmission and plasticity1.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249706033517,"sku":"A-215-10MG-1","price":117.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311547736429,"sku":"A-215-100MG-1","price":846.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311547769197,"sku":"A-215-25MG-1","price":263.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311547801965,"sku":"A-215-5MG-1","price":85.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311547834733,"sku":"A-215-50MG-1","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/A-215_blot.gif?v=1778544407","url":"https:\/\/www.ebiohippo.com\/products\/ana-12-bhb21300008","provider":"BioHippo","version":"1.0","type":"link"}