{"product_id":"ang2-bhn20152510","title":"ANG2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003eANG2\u003c\/strong\u003e, a growth factor construct supplied for antibody validation and cell-based binding assays. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eGrowth factors are secreted or membrane-bound polypeptides that regulate cell proliferation, survival, differentiation, and migration by binding to specific cell-surface receptors and activating downstream signalling cascades (e.g., MAPK\/ERK, PI3K\/AKT, JAK\/STAT). mRNA-based delivery of growth factors enables transient, dose-controlled protein expression in target cells without the need for stable genomic integration. This approach is particularly relevant for ex vivo cell expansion protocols, stem cell differentiation studies, and functional receptor-ligand binding assays, where precise temporal control of growth factor availability is required.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eEx vivo cell manufacturing: Transient mRNA expression of growth factors such as SCF, FLT3L, and TPO is being investigated for short-window stimulation of HSC expansion without chronic cytokine exposure that risks differentiation bias.\u003c\/li\u003e\n  \u003cli\u003eReceptor-ligand interaction studies: Growth factor mRNA provides native-topology ligands for SPR, BLI, and cell-based binding assays validating therapeutic antibody epitopes on receptor ectodomains.\u003c\/li\u003e\n  \u003cli\u003eTissue engineering scaffolds: Growth factor mRNA is incorporated into hydrogel and scaffold matrices to achieve controlled spatiotemporal protein release in tissue-regeneration models.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eReceptor activation assays — transient growth factor mRNA expression stimulates cognate receptors in cell-based reporter assays to characterise ligand-receptor interactions.\u003c\/li\u003e\n  \u003cli\u003eEx vivo cell expansion — short-duration growth factor mRNA pulses to stimulate haematopoietic or stem cell expansion without chronic cytokine exposure.\u003c\/li\u003e\n  \u003cli\u003eAntibody neutralisation studies — mRNA-expressed secreted growth factors as target antigens in neutralisation bioassays validating anti-growth-factor therapeutic antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eSecreted growth factors expressed from transfected cells are present in conditioned media; ensure downstream assays account for the actual secreted protein concentration rather than mRNA input dose.\u003c\/li\u003e\n  \u003cli\u003eGrowth factor receptor expression on the target cell must be confirmed; absence of receptor expression on the transfected cell line may result in undetectable signalling readouts.\u003c\/li\u003e\n  \u003cli\u003eTransient expression windows are typically 24–72 h post-transfection for lyophilised mRNA; titrate dose and time-point empirically for the specific growth factor and cell type.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239631839597,"sku":"IR0065002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239644062061,"sku":"IR0065010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239644094829,"sku":"IR0065020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239644127597,"sku":"IR0065050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239644160365,"sku":"IR0065100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239644193133,"sku":"IR0065500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_fc1cf0c1-852a-4530-9183-4efc3b333b85.png?v=1776677731","url":"https:\/\/www.ebiohippo.com\/products\/ang2-bhn20152510","provider":"BioHippo","version":"1.0","type":"link"}