{"product_id":"anisomycin-bhb21300047","title":"Anisomycin","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e (2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Flagecidin\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 22862-76-6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C14H19NO4.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 0.1-10 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized Powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Natural\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Anisomycin is an antibiotic which inhibits protein synthesis1. It also activates stress-activated protein kinases (SAPK) independent of its ability to block translation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Flagecidin\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eAnisomycin is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis by binding to 60S ribosomal subunits and blocking peptide bond formation, thereby preventing elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2\/p38MAPK), kinase subtypes and p46\/54JNK.6-13Synergizes with growth factors and phorbol esters, anisomycin superinduce c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249704526189,"sku":"A-520-10MG-1","price":42.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311545966957,"sku":"A-520-100MG-1","price":213.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311545999725,"sku":"A-520-25MG-1","price":85.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311546032493,"sku":"A-520-5MG-1","price":31.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311546065261,"sku":"A-520-50MG-1","price":117.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/A-520.gif?v=1778544418","url":"https:\/\/www.ebiohippo.com\/products\/anisomycin-bhb21300047","provider":"BioHippo","version":"1.0","type":"link"}