{"product_id":"annexin-a1-antibody-lipocortin-1-anxa1-bha17135642","title":"Annexin A1 Antibody \/ Lipocortin-1 \/ ANXA1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnnexin A1 Antibody \/ Lipocortin-1 \/ ANXA1 is a anti-A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, nucleus, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e A1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAnnexin A1 antibody detects Annexin A1 (also known as Lipocortin-1 or ANXA1), a calcium-dependent phospholipid-binding protein that mediates anti-inflammatory responses and membrane trafficking. Encoded by the ANXA1 gene on chromosome 9q21.13, this protein belongs to the annexin family characterized by their ability to bind negatively charged phospholipids in a calcium-dependent manner. Annexin A1 contains four annexin repeats and an N-terminal regulatory domain that interacts with phospholipase A2 and formyl peptide receptors. It acts as an effector of glucocorticoid signaling, inhibiting leukocyte transmigration and eicosanoid synthesis to resolve inflammation.\u003cbr\u003e\u003cbr\u003eAnnexin A1 is expressed in many tissues, especially in neutrophils, macrophages, epithelial cells, and the pituitary gland. Upon glucocorticoid stimulation, it translocates to the plasma membrane and is secreted into the extracellular space, where it binds formyl peptide receptor 2 (FPR2\/ALX) on immune cells to inhibit migration and cytokine production. The protein also promotes efferocytosis and tissue repair during inflammation resolution. Dysregulation of Annexin A1 contributes to chronic inflammatory diseases, sepsis, and cancer progression.\u003cbr\u003e\u003cbr\u003eThe Annexin A1 antibody is widely used in immunology, endocrinology, and cancer research to study anti-inflammatory signaling, glucocorticoid response, and cell migration. Western blot analysis typically identifies a 37 kilodalton band corresponding to Annexin A1, while immunohistochemistry reveals cytoplasmic and membrane localization in activated leukocytes and epithelial cells. The antibody is also useful in identifying corticosteroid-mediated gene induction and verifying downstream targets of FPR2 signaling.\u003cbr\u003e\u003cbr\u003eIn cancer biology, Annexin A1 can act as a context-dependent modulator of tumor invasion and metastasis. It regulates cytoskeletal remodeling, apoptosis, and vesicle trafficking, influencing tumor cell motility. The Annexin A1 antibody helps delineate these pathways by enabling accurate detection in tumor and immune microenvironment studies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=A1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=A1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=A1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/A1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=A1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298687341,"sku":"FY12739","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_12cabe08-7d8b-4efe-8e02-185c66241a8f.jpg?v=1772019381","url":"https:\/\/www.ebiohippo.com\/products\/annexin-a1-antibody-lipocortin-1-anxa1-bha17135642","provider":"BioHippo","version":"1.0","type":"link"}