{"product_id":"annexin-v-egfp-pi-apoptosis-detection-kit-bht20800103","title":"Annexin V-EGFP\/PI Apoptosis Detection Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-444b0c7e=\"\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-444b0c7e=\"\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAnnexin V-EGFP\/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis.\u003cbr data-mce-fragment=\"1\"\u003eThe detection principle is as follows: in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V is a Ca\u003csup data-mce-fragment=\"1\"\u003e2+\u003c\/sup\u003e -dependent phospholipid-binding protein with a molecular weight of 35 to 36 kDa that binds to PS with high affinity. The phosphatidyl serine can bind to the membrane of early apoptotic cells through the exposed phosphatidyl serine on the outside of the cell.\u003cbr data-mce-fragment=\"1\"\u003eIn addition, Propidium Iodide (PI) is provided to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye, which can not pass through the intact cell membrane of normal cells or early apoptotic cells but can pass through the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Thus, when Annexin V was combined with PI, PI was excluded from living cells (Annexin V\u003csup data-mce-fragment=\"1\"\u003e-\u003c\/sup\u003e\/PI\u003csup data-mce-fragment=\"1\"\u003e-\u003c\/sup\u003e) and early apoptotic cells (Annexin V\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e\/PI\u003csup data-mce-fragment=\"1\"\u003e-\u003c\/sup\u003e). The late apoptotic and necrotic cells were double positive for both EGFP and PI (Annexin V\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e\/PI\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e).\u003cbr data-mce-fragment=\"1\"\u003eThis kit is suitable for flow cytometry and fluorescence microscopy.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Components\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\" data-mce-fragment=\"1\"\u003e\n\u003ctable cellspacing=\"0\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponent\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303ES20（20 T）\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303ES50（50 T）\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303ES60（100 T）\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303-A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eAnnexin V-EGFP\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e0.1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e0.25 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e0.5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303-B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003ePI Staining Solution\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e0.2 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e0.5 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40303-C\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1×Binding Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e25 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e50 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe components are shipped with an ice pack and can be stored at -20°C keep out of light, and avoid repeated freezing and thawing for 1 year.\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e[Notes]: If it needs to be used repeatedly in a short period, it can be stored at 4 ℃ and protected from light for half a year.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCautions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1) Since apoptosis is a rapid process, it is recommended that samples be analyzed within 1 hour after staining.\u003cbr data-mce-fragment=\"1\"\u003e2) For adherent cells, digestion is a critical step. Do not use EDTA in the digestive solution as EDTA can affect the binding of Annexin V to PS.\u003cbr data-mce-fragment=\"1\"\u003e3) Annexin V-FITC, but not Annexin V-EGFP, should be used to fix cells, such as to detect apoptosis and cell cycle at the same time, because EGFP would be denatured and lose its ability to excite fluorescence during fixation. Cells were incubated with Annexin V-FITC before fixation and unbound Annexin V-FITC was washed away with Binding Buffer. Because increased cell permeability during fixation creates cell debris that can bind to Annexin V and interfere with the results.\u003cbr data-mce-fragment=\"1\"\u003e4) If the sample is from blood, be sure to remove platelets from the blood. Because platelets contain PS, which can bind to Annexin V, interfering with the results. Platelets can be washed away by using a buffering agent containing EDTA and centrifuging at 200 g.\u003cbr data-mce-fragment=\"1\"\u003e5) Please centrifuge the reagent briefly before opening the cover, and throw the liquid on the inner wall of the cover to the bottom of the tube to avoid liquid sprinkling when opening the cover.\u003cbr data-mce-fragment=\"1\"\u003e6) Annexin V-EGFP and PI are photosensitive substances, so please avoid light during operation.\u003cbr data-mce-fragment=\"1\"\u003e7) For research use only! \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eExperiment design\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eBlank tube: Negative control cells without Annexin V-EGFP and PI Staining Solution were used for voltage regulation.\u003cbr data-mce-fragment=\"1\"\u003eSingle stained tubes: Positive control cells, supplemented only with Annexin V-EGFP, were used to modulate compensation.\u003cbr data-mce-fragment=\"1\"\u003eDetecting tube: The cells were treated with Annexin V-EGFP and PI Staining Solution. The experimental data were obtained by adjusting the parameters of the blank tube and single dye tube.\u003cbr data-mce-fragment=\"1\"\u003e1.1 Sample dyeing\u003cbr data-mce-fragment=\"1\"\u003e1) suspension cell:  Cells were collected by centrifugation at 300 g for 5 min at 4 ° C.\u003cbr data-mce-fragment=\"1\"\u003e   adherent cell: After digestion with trypsin without EDTA, cells were harvested by centrifugation at 300 g for 5 min at 4 ° C. Trypsin digestion time should not be too long to prevent false positives.\u003cbr data-mce-fragment=\"1\"\u003e2) The cells were washed with precooled PBS twice, each time at 300 g, and centrifuged at 4 ℃ for 5 min.\u003cbr data-mce-fragment=\"1\"\u003e3) The cells were resuspended with 1×Binding Buffer and the concentration was adjusted to 1~5 ×106\/mL.\u003cbr data-mce-fragment=\"1\"\u003e4) 100 μL of cell suspension was added into 5 mL flow cytometry tubes, 5 μl Annexin V-EGFP was added, and the cells were mixed and incubated for 5 min at room temperature under the light.\u003cbr data-mce-fragment=\"1\"\u003e5) Adding 10 μl PI Solution and 400 μl PBS, the Staining was performed immediately.\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e[Notes]: When using flow cytometry to detect apoptosis, PI is greatly affected by time, and too long time will lead to an increase in PI staining, so flow cytometry should be completed within 1 h.\u003c\/span\u003e\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1.2 Flow cytometry analysis\u003cbr data-mce-fragment=\"1\"\u003eThe maximum excitation wavelength of EGFP was 488 nm, and the maximum emission wavelength was 507 nm; The maximum excitation wavelength of the PI-DNA complex was 535 nm, and the maximum emission wavelength was 615 nm. Software such as CellQuest was used for analysis and a two-color dot plot was drawn, EGFP is the abscissa and PI is the ordinate. Collect 10,000 events per sample. In a typical experiment, the cells can be divided into three subgroups, living cells only have very low-intensity background fluorescence, early apoptotic cells only have strong green fluorescence, and late apoptotic cells have green and red fluorescence double staining.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"top_tabc\" data-mce-fragment=\"1\"\u003e\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox1\" class=\"boxTap\" data-mce-fragment=\"1\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-444b0c7e=\"\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-v-444b0c7e=\"\" class=\"cwrap\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\" data-mce-fragment=\"1\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003cp data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\u003ca title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40303-MSDS-HB250721.pdf?v=1753171155\"\u003e40303_MSDS_HB250721.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/40303-B-Annexin%20V-EGFP%20PI%20Apoptosis%20Detection%20Kit-MSDS-HB220804.pdf\" download=\"\" title=\"download\" class=\"down\" data-mce-fragment=\"1\" target=\"_blank\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"list\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-v-98c835fc=\"\" class=\"title\" data-mce-fragment=\"1\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\n\u003cp data-v-98c835fc=\"\" data-mce-fragment=\"1\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40303-Manual-Ver.EN20250618_594d2873-b618-4576-86a7-53a360ac3fff.pdf?v=1753171214\" target=\"_blank\"\u003e40303_Manual_Ver.EN20260116.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53339939406189,"sku":"40303ES20","price":230.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53339942650221,"sku":"40303ES50","price":420.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339942682989,"sku":"40303ES60","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/41304_46394d0c-718b-4644-9050-b651cf309a7c.png?v=1782159261","url":"https:\/\/www.ebiohippo.com\/products\/annexin-v-egfp-pi-apoptosis-detection-kit-bht20800103","provider":"BioHippo","version":"1.0","type":"link"}