{"product_id":"annexin-v-fitc-pi-apoptosis-detection-kit-bht20800102","title":"Annexin V-FITC\/PI Apoptosis Detection Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n\u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n\u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"card__section\"\u003e\n\u003cdiv class=\"rte text--pull\"\u003e\n\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cstyle\u003e\n.bhd-desc .bhd-lead{font-size:15px;line-height:1.65}\n.bhd-desc .bhd-facts{display:grid;grid-template-columns:repeat(auto-fit,minmax(150px,1fr));gap:10px;margin:18px 0}\n.bhd-desc .bhd-fact{border:1px solid #e6eaee;border-left:3px solid #1f6f3f;border-radius:8px;padding:10px 12px;background:#fafcfb}\n.bhd-desc .bhd-fact b{display:block;font-size:11px;letter-spacing:.04em;text-transform:uppercase;color:#6b7b73;margin-bottom:3px;font-weight:600}\n.bhd-desc .bhd-fact span{font-size:14px;color:#1f3b2c;font-weight:600}\n\u003c\/style\u003e\n\u003cdiv class=\"bhd-desc\"\u003e\n\u003cp class=\"bhd-lead\"\u003eThe \u003cstrong\u003eAnnexin V-FITC\/PI Apoptosis Detection Kit\u003c\/strong\u003e is a ready-to-use flow cytometry assay for quantifying apoptosis in live, intact cells. By pairing FITC-conjugated Annexin V with propidium iodide (PI), it resolves viable, early-apoptotic, and late-apoptotic\/necrotic populations in a single stain — a reliable workflow for apoptosis detection by flow cytometry or fluorescence microscopy.\u003c\/p\u003e\n\u003cdiv class=\"bhd-facts\"\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eFormat\u003c\/b\u003e\u003cspan\u003eLiquid, ready-to-use\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eDetection\u003c\/b\u003e\u003cspan\u003eFlow cytometry \/ microscopy\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eFluorophores\u003c\/b\u003e\u003cspan\u003eFITC (Ex 488 nm) + PI\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eKit sizes\u003c\/b\u003e\u003cspan\u003e20 \/ 50 \/ 100 tests\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eStorage\u003c\/b\u003e\u003cspan\u003e−20 °C\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhd-fact\"\u003e\n\u003cb\u003eGrade\u003c\/b\u003e\u003cspan\u003eResearch Use Only\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eHow Annexin V\/PI staining detects apoptosis\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eIn healthy cells, phosphatidylserine (PS) faces the inner leaflet of the plasma membrane. Early in apoptosis, PS translocates to the cell surface before membrane integrity is lost. Annexin V is a Ca\u003csup\u003e2+\u003c\/sup\u003e-dependent phospholipid-binding protein (35–36 kDa) with high affinity for PS, so FITC-labeled Annexin V binds externalized PS and identifies early-apoptotic cells.\u003c\/p\u003e\n\u003cp\u003ePropidium iodide (PI) cannot cross the intact membrane of viable or early-apoptotic cells, but enters once membrane integrity is lost, staining the nuclei of late-apoptotic and necrotic cells. Used together, Annexin V and PI separate four populations in a single measurement: viable (Annexin V−\/PI−), early apoptotic (Annexin V+\/PI−), late apoptotic or necrotic (Annexin V+\/PI+), and damaged or necrotic (Annexin V−\/PI+).\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eKey features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAll reagents supplied in ready-to-use liquid form — no reconstitution required.\u003c\/li\u003e\n\u003cli\u003eValidated for both flow cytometry and fluorescence microscopy.\u003c\/li\u003e\n\u003cli\u003eClear four-quadrant resolution of viable, early-apoptotic, and late-apoptotic\/necrotic cells.\u003c\/li\u003e\n\u003cli\u003eBright, well-separated FITC and PI signals for straightforward gating and imaging.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eResult interpretation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eStaining pattern\u003c\/td\u003e\n\u003ctd\u003eCell population\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnexin V− \/ PI−\u003c\/td\u003e\n\u003ctd\u003eViable cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnexin V+ \/ PI−\u003c\/td\u003e\n\u003ctd\u003eEarly apoptotic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnexin V+ \/ PI+\u003c\/td\u003e\n\u003ctd\u003eLate apoptotic \/ necrotic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnexin V− \/ PI+\u003c\/td\u003e\n\u003ctd\u003eNecrotic or mechanically damaged cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eAnnexin V\/PI staining protocol (flow cytometry)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eHarvest cells gently (Accutase is preferred over trypsin) and avoid mechanical stress. Collect approximately 1–5 × 10\u003csup\u003e5\u003c\/sup\u003e cells per sample.\u003c\/li\u003e\n\u003cli\u003eWash cells twice with cold PBS, then resuspend in 1× Binding Buffer.\u003c\/li\u003e\n\u003cli\u003eAdd Annexin V-FITC and PI Staining Solution according to the kit manual (typically 5 µL Annexin V-FITC and 5–10 µL PI per test); mix gently.\u003c\/li\u003e\n\u003cli\u003eIncubate for 10–15 min at room temperature, protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 1× Binding Buffer and analyze by flow cytometry within 1 hour (488 nm excitation; FITC detected at ~530 nm, PI at ~617 nm). Do not fix cells before Annexin V staining.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eInclude unstained, Annexin V-only, and PI-only single-stain controls to set compensation and gating. Refer to the product manual for the full validated protocol.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eApoptosis assay comparison\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMethod\u003c\/td\u003e\n\u003ctd\u003eWhat it detects\u003c\/td\u003e\n\u003ctd\u003eBest suited for\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnexin V \/ PI (this kit)\u003c\/td\u003e\n\u003ctd\u003ePhosphatidylserine externalization (early) and loss of membrane integrity (late)\u003c\/td\u003e\n\u003ctd\u003eLive, intact cells by flow cytometry or fluorescence microscopy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTUNEL\u003c\/td\u003e\n\u003ctd\u003eDNA fragmentation (late apoptosis)\u003c\/td\u003e\n\u003ctd\u003eFixed cells and tissue sections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCaspase-3\/7 activity\u003c\/td\u003e\n\u003ctd\u003eEffector caspase activation\u003c\/td\u003e\n\u003ctd\u003eMechanistic confirmation of the apoptotic pathway\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-FITC \/ PI apoptosis detection (flow cytometry, fluorescence microscopy)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection targets\u003c\/td\u003e\n\u003ctd\u003eViable, early-apoptotic, and late-apoptotic \/ necrotic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFluorophores\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-FITC (Ex 488 nm \/ Em ~530 nm); propidium iodide (Em ~617 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eLive, intact cultured cells (single-cell suspension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit sizes\u003c\/td\u003e\n\u003ctd\u003e20 T \/ 50 T \/ 100 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePacking form\u003c\/td\u003e\n\u003ctd\u003eBottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eDry ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd\u003e12 months under recommended storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrade\u003c\/td\u003e\n\u003ctd\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eKit components\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e40302ES20 (20T)\u003c\/td\u003e\n\u003ctd\u003e40302ES50 (50T)\u003c\/td\u003e\n\u003ctd\u003e40302ES60 (100T)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e40302-A\u003c\/td\u003e\n\u003ctd\u003eAnnexin V-FITC\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e40302-B\u003c\/td\u003e\n\u003ctd\u003ePI Staining Solution\u003c\/td\u003e\n\u003ctd\u003e200 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003ctd\u003e1.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e40302-C\u003c\/td\u003e\n\u003ctd\u003e1× Binding Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage \u0026amp; shipping\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eShipped on dry ice. Store at −15 °C to −25 °C (−20 °C nominal); stable for 1 year under recommended conditions. Aliquot to minimize repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Annexin V-FITC\/PI apoptosis detection kit flow cytometry result\" src=\"https:\/\/seas.ysbuy.com\/file\/80130\/4bd0-168743392878824619.jpg\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53339940094317,"sku":"40302ES20","price":230.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53339941142893,"sku":"40302ES50","price":420.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339941175661,"sku":"40302ES60","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/annexin-pi-mechanism-schematic.png?v=1782504681","url":"https:\/\/www.ebiohippo.com\/products\/annexin-v-fitc-pi-apoptosis-detection-kit-bht20800102","provider":"BioHippo","version":"1.0","type":"link"}