{"product_id":"annexin-v-ysfluor-488-pi-apoptosis-detection-kit-bht20800105","title":"Annexin V-YSFluor™ 488\/PI Apoptosis Detection Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv data-v-444b0c7e=\"\" class=\"top_tabc proDetail\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp align=\"justify\"\u003eThe Annexin V-YSFluor™ 488\/PI Apoptosis Detection Kit utilizes Annexin V conjugated to YSFluor™ 488 as a fluorescent probe to detect early-stage apoptosis. The assay can be analyzed by flow cytometry or other fluorescence detection platforms.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003ePrinciple of Detection:In healthy, viable cells, phosphatidylserine (PS) is localized to the inner leaflet of the plasma membrane. During early apoptosis, PS is translocated to the outer surface of the cell membrane, becoming exposed to the extracellular environment. Annexin V is a 35–36 kDa Ca²⁺-dependent phospholipid-binding protein with high affinity for PS. Fluorescently labeled Annexin V-YSFluor™ 488 binds specifically to PS exposed on the surface of early apoptotic cells.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThis kit also includes Propidium Iodide (PI), a nucleic acid dye used to distinguish early apoptotic cells from late apoptotic or necrotic cells. PI is membrane-impermeant and cannot enter live or early apoptotic cells with intact membranes. However, it readily penetrates cells with compromised membranes—such as late apoptotic or necrotic cells—and stains their nuclei red.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eWhen used together: Viable cells: Annexin V⁻ \/ PI⁻; Early apoptotic cells: Annexin V⁺ \/ PI⁻; Late apoptotic or necrotic cells: Annexin V⁺ \/ PI⁺ (dual-positive).\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRapid and reliable detection: Enables fast and accurate discrimination between live, early apoptotic, late apoptotic, and necrotic cells.\u003c\/li\u003e\n\u003cli\u003eDual-color staining: Combines Annexin V-YSFluor™ 488 for early apoptosis (green) and propidium iodide (PI) for late apoptosis or necrosis (red).\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity and low background: Provides clear, distinct population separation in flow cytometry or fluorescence microscopy.\u003c\/li\u003e\n\u003cli\u003eWide compatibility: Applicable to both adherent and suspension cells from various mammalian species.\u003c\/li\u003e\n\u003cli\u003eSimple protocol: One-step staining procedure without cell fixation, preserving cell morphology and viability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\" valign=\"top\"\u003e\n\u003cp\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"332\" valign=\"top\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"top\"\u003e\n\u003cp\u003e40305ES20\u003c\/p\u003e\n\u003cp\u003e(20 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"top\"\u003e\n\u003cp\u003e40305ES50\u003c\/p\u003e\n\u003cp\u003e(50 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"top\"\u003e\n\u003cp\u003e40305ES60\u003c\/p\u003e\n\u003cp\u003e(100 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\" valign=\"center\"\u003e\n\u003cp\u003e40305-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"332\" valign=\"center\"\u003e\n\u003cp\u003eAnnexin V-YSFluor™ 488\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\" valign=\"center\"\u003e\n\u003cp\u003e40305-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"332\" valign=\"center\"\u003e\n\u003cp\u003ePI Staining Solution (20 μg\/mL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e1.0 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"189\" valign=\"center\"\u003e\n\u003cp\u003e40305-C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"332\" valign=\"center\"\u003e\n\u003cp\u003e1× Binding Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e10 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e25 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"154\" valign=\"center\"\u003e\n\u003cp\u003e50 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25℃~-15℃ for one year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/40305-A-Annexin%20V-Alexa%20Fluor488%20Apoptosis%20detection%20kit-MSDS-HB220804.pdf\" download=\"\" title=\"download\" target=\"_blank\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e40305-A-Annexin V-Alexa Fluor488 Apoptosis detection kit-MSDS-HB220804.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/40305-B-Annexin%20V-Alexa%20Fluor488%20Apoptosis%20detection%20kit-MSDS-HB220804.pdf\" download=\"\" title=\"download\" target=\"_blank\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e40305-B-Annexin V-Alexa Fluor488 Apoptosis detection kit-MSDS-HB220804.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\u003ca data-v-98c835fc=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/40305-C-Annexin%20V-Alexa%20Fluor488%20Apoptosis%20detection%20kit-MSDS-HB220804.pdf\" download=\"\" title=\"download\" target=\"_blank\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e40305-C-Annexin V-Alexa Fluor488 Apoptosis detection kit-MSDS-HB220804.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40305ES-Manual-Ver.EN20251111.pdf?v=1762926441\"\u003e40305_Manual_Ver.EN20251111.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003ePublications Using This Product\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e[1] Zhang M, Weng Y, Cao Z, et al. ROS-Activatable siRNA-Engineered Polyplex for NIR-Triggered Synergistic Cancer Treatment. ACS Appl Mater Interfaces. 2020;12(29):32289-32300. doi:10.1021\/acsami.0c06614(IF:8.758)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[2] Duan Z , Luo Q , Gu L , et al. A co-delivery nanoplatform for a lignan-derived compound and perfluorocarbon tuning IL-25 secretion and the oxygen level in tumor microenvironments for meliorative tumor radiotherapy. Nanoscale. 2021;13(32):13681-13692. doi:10.1039\/d1nr03738b(IF:7.790)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[3] Tang L, Zhang C, Lu L, et al. Melatonin Maintains Inner Blood-Retinal Barrier by Regulating Microglia via Inhibition of PI3K\/Akt\/Stat3\/NF-κB Signaling Pathways in Experimental Diabetic Retinopathy. Front Immunol. 2022;13:831660. Published 2022 Mar 15. doi:10.3389\/fimmu.2022.831660(IF:7.561)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[4] Xie H, Zhang C, Liu D, et al. Erythropoietin protects the inner blood-retinal barrier by inhibiting microglia phagocytosis via Src\/Akt\/cofilin signalling in experimental diabetic retinopathy. Diabetologia. 2021;64(1):211-225. doi:10.1007\/s00125-020-05299-x(IF:7.518)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[5] Yue C, Yang Y, Song J, et al. Mitochondria-targeting near-infrared light-triggered thermosensitive liposomes for localized photothermal and photodynamic ablation of tumors combined with chemotherapy. Nanoscale. 2017;9(31):11103-11118. doi:10.1039\/c7nr02193c(IF:7.367)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[6] Yu H, Yang X, Xiao X, et al. Human Adipose Mesenchymal Stem Cell-derived Exosomes Protect Mice from DSS-Induced Inflammatory Bowel Disease by Promoting Intestinal-stem-cell and Epithelial Regeneration. Aging Dis. 2021;12(6):1423-1437. Published 2021 Sep 1. doi:10.14336\/AD.2021.0601(IF:6.745)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[7] Huang J, Liu Y, Chen JX, et al. Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy. Acta Pharmacol Sin. 2022;43(1):50-63. doi:10.1038\/s41401-021-00639-y(IF:6.150)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[8] Wang X, Zhang R, Wu T, et al. Successive treatment with naltrexone induces epithelial-mesenchymal transition and facilitates the malignant biological behaviors of bladder cancer cells. Acta Biochim Biophys Sin (Shanghai). 2021;53(2):238-248. doi:10.1093\/abbs\/gmaa169(IF:3.848)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[9] Qu B, Han X, Zhao L, Zhang F, Gao Q. Relationship of HIF‑1α expression with apoptosis and cell cycle in bone marrow mesenchymal stem cells from patients with myelodysplastic syndrome. Mol Med Rep. 2022;26(1):239. doi:10.3892\/mmr.2022.12755(IF:2.952)\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e[10] Fan W, Gao X, Ding C, et al. Estrogen receptors participate in carcinogenesis signaling pathways by directly regulating NOD-like receptors. Biochem Biophys Res Commun. 2019;511(2):468-475. doi:10.1016\/j.bbrc.2019.02.085(IF:2.705)\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e[11] \u003c!--[endif]--\u003eFeng Z, Zhu Z, Chen W, Bai Y, Hu D, Cheng J. Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis. Clin Proteomics. 2020;17:32. Published 2020 Sep 5. doi:10.1186\/s12014-020-09295-6(IF:2.568)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53339939111277,"sku":"40305ES20","price":240.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53339940553069,"sku":"40305ES50","price":440.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339940585837,"sku":"40305ES60","price":640.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/41304_e3bf34f2-5ad3-49f9-b209-f33374d718e4.png?v=1782159261","url":"https:\/\/www.ebiohippo.com\/products\/annexin-v-ysfluor-488-pi-apoptosis-detection-kit-bht20800105","provider":"BioHippo","version":"1.0","type":"link"}