{"product_id":"anti-6-his-tag-hhhhhh-antibody-as01-bha21409452","title":"Anti-6*His Tag (HHHHHH) Antibody (AS01)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-6*His Tag (HHHHHH) Antibody (AS01) is intended for detection of \u003cstrong\u003e6*His Tag\u003c\/strong\u003e. Commonly used in IF, IP, WB applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Validate antibody specificity by WB with KD\/KO control lysates, Detect target localization by IF\/ICC in cultured cells, Enrich target by IP\/Co-IP from cell lysates for downstream analysis, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e 6*HIS, 6xHis, 6HIS, HIS.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a, kappa; Purification: Protein A\/G purified from cell culture supernatant.; Format: Liquid.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003e6*His Tag (6*HIS) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular \u0026amp; Cellular Biology. Common alternative names and symbols used in the literature include: 6xHis, 6HIS, HIS. Based on the annotated name\/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, include fresh protease inhibitors (and phosphatase inhibitors for PTM targets) and verify the dominant band at the expected size; additional bands may reflect isoforms, processing, or cross-reactivity.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037061734765,"sku":"YP205023-50UG","price":128.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037790560621,"sku":"YP205023-100UG","price":218.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YP205023-SDSPAGE-1.jpg?v=1771748299","url":"https:\/\/www.ebiohippo.com\/products\/anti-6-his-tag-hhhhhh-antibody-as01-bha21409452","provider":"BioHippo","version":"1.0","type":"link"}