{"product_id":"anti-aag-mpg-antibody-picoband-bha21004465","title":"Anti-AAG\/MPG Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-AAG\/MPG Antibody Picoband® is an antibody reagent for detection of \u003cstrong\u003eMPG\u003c\/strong\u003e (Signal transducer and activator of transcription 1-alpha\/beta). Researchers commonly use anti-MPG antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-AAG\/MPG Antibody Picoband® catalog # A00037. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MPG — Signal transducer and activator of transcription 1-alpha\/beta (Signal transducer and activator of transcription 1-alpha\/beta). Alternative names: Signal transducer and activator of transcription 1-alpha\/beta;Transcription factor ISGF-3 components p91\/p84;STAT1;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Polyclonal; Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies context:\u003c\/strong\u003e Host: Rabbit, Reactivity: Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Immunogen affinity purified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e E.coli-derived human AAG\/MPG recombinant protein (Position: Q20-A298).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e observed 37 kDa, calculated 87335 MW (reported)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProvided application(s):\u003c\/strong\u003e WB, IHC, Flow, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThese attributes help contextualize how the antibody is commonly selected (host\/clonality\/isotype\/label) and how signals are interpreted across sample types and assay formats.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eFunction:\u003c\/strong\u003e Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG\/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G\/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG\/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. .\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Cytoplasm . Nucleus . Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details:\u003c\/strong\u003e In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse\/human and hamster\/human hybrid cell lines.TheMPGgene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized humanMPGgene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eQuantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.\u003c\/li\u003e \u003cli\u003eIsoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.\u003c\/li\u003e \u003cli\u003eContext-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e compare relative target abundance and apparent size shifts (e.g., isoforms\/PTMs) across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e assess distribution across tissue compartments and compare staining patterns between groups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and compare shifts after stimulation or differentiation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAcross these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eApparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.\u003c\/li\u003e \u003cli\u003eSpecies reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.\u003c\/li\u003e \u003cli\u003eControl concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO\/KD, peptide competition, or independent assays) to support interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eFor antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProt entry for MPG (P29372) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P29372 - Ensembl gene summary for MPG — Ensembl — https:\/\/www.ensembl.org - HGNC gene symbol report for MPG — HGNC — https:\/\/www.genenames.org - Antibody validation concepts (general) — NIH\/NCBI resources — https:\/\/www.ncbi.nlm.nih.gov --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53068599001453,"sku":"A00037","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53068829753709,"sku":"A00037-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53068829786477,"sku":"A00037-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53068829819245,"sku":"A00037-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53068829852013,"sku":"A00037-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53068829884781,"sku":"A00037-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53068829917549,"sku":"A00037-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53068829950317,"sku":"A00037-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53068829983085,"sku":"A00037-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53068830015853,"sku":"A00037-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53068830048621,"sku":"A00037-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53068830081389,"sku":"A00037-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a00037-mpg-primary-antibodies-wb-testing-1.jpg?v=1772611325","url":"https:\/\/www.ebiohippo.com\/products\/anti-aag-mpg-antibody-picoband-bha21004465","provider":"BioHippo","version":"1.0","type":"link"}