{"product_id":"anti-adam12-polyclonal-antibody-bha21402011","title":"Anti-ADAM12 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eThis antibody is intended for research detection of \u003cstrong\u003eADAM12\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eADAM12 (ADAM 12)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eADAM12 is a member of the ADAM (a disintegrin and metalloproteinase) family that can influence cell–cell and cell–matrix interactions and proteolytic processing of membrane proteins. It has been studied in myogenesis, tissue remodeling\/fibrosis, and cancer where altered ADAM12 expression may correlate with invasive or remodeling phenotypes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003emembrane-associated (may vary by cell type and experimental conditions).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eCommonly used in \u003cstrong\u003eMolecular \u0026amp; Cellular Biology\u003c\/strong\u003e workflows to study expression changes, pathway state, and cellular localization of ADAM12.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible workflows\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eELISA\u003c\/li\u003e\n\u003cli\u003eIHC\u003c\/li\u003e\n\u003cli\u003eWB\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eWestern blot: optimize sample preparation (reducing vs. non‑reducing and denaturing conditions) and titrate antibody to balance sensitivity and background. Apparent molecular weight may vary with isoforms and post‑translational modifications (e.g., glycosylation or phosphorylation).\u003c\/li\u003e\n\u003cli\u003eIHC: epitope accessibility can depend strongly on fixation and antigen retrieval. Test heat‑induced retrieval buffers (citrate pH ~6 or Tris‑EDTA pH ~9) and adjust retrieval time to improve signal-to-background.\u003c\/li\u003e\n\u003cli\u003eELISA: performance is sensitive to coating conditions and blocking chemistry; optimize capture density and blocking reagent to reduce non‑specific binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eRecommended controls include a positive sample reported to express the target (literature‑validated cell line or tissue), a negative\/low‑expression sample when available, and ideally genetic knockdown\/knockout or peptide‑blocking approaches to confirm specificity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eE. coli - derived recombinant Human ADAM12 (Val31-His350).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003cbr\u003eFor research use only. Not for diagnostic procedures.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036812140909,"sku":"HT428014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037394493805,"sku":"HT428014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HT428014-abinscience-WB-1.jpg?v=1771743795","url":"https:\/\/www.ebiohippo.com\/products\/anti-adam12-polyclonal-antibody-bha21402011","provider":"BioHippo","version":"1.0","type":"link"}