{"product_id":"anti-asic2a-antibody-bha21301231","title":"Anti-ASIC2a Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-ASIC2a Antibody is an antibody targeting ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IHC, IP, WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG (also reported as ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Intracellular, N-terminus.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Rat, mouse - identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecificity statement (as provided):\u003c\/strong\u003e The antibody is specific for ASIC2a and will not recognize ASIC2b..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eKO-validated:\u003c\/strong\u003e yes (validation context may be assay-dependent).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin\/epithelial Na+ channels (DEG\/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eMapping receptor\/channel localization across neuronal subtypes and subcellular compartments.\u003c\/li\u003e   \u003cli\u003eLinking trafficking or surface expression changes to activity-dependent signaling and plasticity.\u003c\/li\u003e   \u003cli\u003eUsing KO\/KD or blocking-peptide concepts to strengthen antibody-based target assignment.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e   \u003cli\u003eImmunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence\/ICC: assess subcellular localization and co-localization with markers in cells or sections.\u003c\/li\u003e   \u003cli\u003eImmunoprecipitation (IP): enrich the target for downstream detection or complex analysis (context-dependent).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eConceptual control: KO\/KD samples provide orthogonal support for target assignment when available.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: BLP-SC012.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-SC012; Negative control: BLP-SC012.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"0.2 ml (Carrier Free) \/ 1","offer_id":53064855486829,"sku":"ASC-012-CF-0P2ML-1","price":994.0,"currency_code":"USD","in_stock":true},{"title":"0.2 ml \/ 1","offer_id":53064976564589,"sku":"ASC-012-0P2ML-1","price":795.0,"currency_code":"USD","in_stock":true},{"title":"25 mcl \/ 1","offer_id":53064976597357,"sku":"ASC-012-25MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064976630125,"sku":"ASC-012-50MCL-1","price":697.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ASC-012_ept.gif?v=1772461082","url":"https:\/\/www.ebiohippo.com\/products\/anti-asic2a-antibody-bha21301231","provider":"BioHippo","version":"1.0","type":"link"}