{"product_id":"anti-atf6-polyclonal-antibody-bha21403175","title":"Anti-ATF6 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eThis antibody is intended for research detection of \u003cstrong\u003eATF6\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eATF6 (ATF6-alpha)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eATF6 is a transcriptional regulator that influences gene expression programs. Transcription factors integrate developmental, metabolic, and stress cues to control lineage specification, cell-cycle progression, and cellular identity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003enuclear (may vary by cell type and experimental conditions).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eCommonly used in \u003cstrong\u003eMolecular \u0026amp; Cellular Biology\u003c\/strong\u003e workflows to study expression changes, pathway state, and cellular localization of ATF6.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible workflows\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eELISA\u003c\/li\u003e\n\u003cli\u003eIHC\u003c\/li\u003e\n\u003cli\u003eWB\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eWestern blot: optimize sample preparation (reducing vs. non‑reducing and denaturing conditions) and titrate antibody to balance sensitivity and background. Apparent molecular weight may vary with isoforms and post‑translational modifications (e.g., glycosylation or phosphorylation).\u003c\/li\u003e\n\u003cli\u003eIHC: epitope accessibility can depend strongly on fixation and antigen retrieval. Test heat‑induced retrieval buffers (citrate pH ~6 or Tris‑EDTA pH ~9) and adjust retrieval time to improve signal-to-background.\u003c\/li\u003e\n\u003cli\u003eELISA: performance is sensitive to coating conditions and blocking chemistry; optimize capture density and blocking reagent to reduce non‑specific binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eRecommended controls include a positive sample reported to express the target (literature‑validated cell line or tissue), a negative\/low‑expression sample when available, and ideally genetic knockdown\/knockout or peptide‑blocking approaches to confirm specificity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eE. coli - derived recombinant Human ATF6 (Ala547-Ser632).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003cbr\u003eFor research use only. Not for diagnostic procedures.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036852543853,"sku":"HB163024-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037432209773,"sku":"HB163024-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB163024-abinscience-WB-1.jpg?v=1771744535","url":"https:\/\/www.ebiohippo.com\/products\/anti-atf6-polyclonal-antibody-bha21403175","provider":"BioHippo","version":"1.0","type":"link"}