{"product_id":"anti-barzolvolimab-antibody-elisa-kit-bhe10814181","title":"Anti-Barzolvolimab antibody ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Anti-Barzolvolimab antibody ELISA Kit measures antibodies against Barzolvolimab in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antigen sandwich format: antigen pre-coated on the plate captures anti-Barzolvolimab antibody from the sample, and a biotin-labeled detection antigen then binds the captured antibody. HRP-Streptavidin and TMB substrate generate a color signal read at 450 nm, and concentration is calculated from the standard curve.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAlias\u003c\/th\u003e\n\u003ctd\u003eAnti-Barzolvolimab Ab ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eUniversal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA, Double antigen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eOD450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction Duration\u003c\/th\u003e\n\u003ctd\u003e4 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRange\u003c\/th\u003e\n\u003ctd\u003e1.563-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.938 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e2-8°C (Sealed), Don't cryopreserve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eSpecifically binds with Anti-Barzolvolimab Ab, no obvious cross reaction with other analogues.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eCode\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE001\u003c\/td\u003e\n\u003ctd\u003eELISA Microplate (dismountable)\u003c\/td\u003e\n\u003ctd\u003e8 × 12 strips\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE002\u003c\/td\u003e\n\u003ctd\u003eLyophilized Standard\u003c\/td\u003e\n\u003ctd\u003e2 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE003\u003c\/td\u003e\n\u003ctd\u003eBiotin-labeled Antigen (100X)\u003c\/td\u003e\n\u003ctd\u003e120 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE034\u003c\/td\u003e\n\u003ctd\u003eHRP-Streptavidin Conjugate (SABC, 100X)\u003c\/td\u003e\n\u003ctd\u003e120 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE024\u003c\/td\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE039\u003c\/td\u003e\n\u003ctd\u003eSample Dilution Buffer\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE040\u003c\/td\u003e\n\u003ctd\u003eAntigen Dilution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE049\u003c\/td\u003e\n\u003ctd\u003eSABC Dilution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE026\u003c\/td\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE038\u003c\/td\u003e\n\u003ctd\u003eWash Buffer (25X)\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE006\u003c\/td\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e5 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE007\u003c\/td\u003e\n\u003ctd\u003eProduct Description\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecovery\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSerum\u003c\/th\u003e\n\u003ctd\u003e90-102% (average 97%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEDTA plasma\u003c\/th\u003e\n\u003ctd\u003e88-105% (average 95%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHeparin plasma\u003c\/th\u003e\n\u003ctd\u003e87-102% (average 93%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eIntra-assay CV\u003c\/th\u003e\n\u003ctd\u003e4.69-4.95% (3 samples, 3.03-49.17 ng\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInter-assay CV\u003c\/th\u003e\n\u003ctd\u003e4.82-6.57% (3 samples, 3.1-50.48 ng\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eLinearity (recovery after dilution)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSerum\u003c\/th\u003e\n\u003ctd\u003e1:2 86-105%; 1:4 82-100%; 1:8 80-95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEDTA plasma\u003c\/th\u003e\n\u003ctd\u003e1:2 85-105%; 1:4 83-95%; 1:8 80-89%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHeparin plasma\u003c\/th\u003e\n\u003ctd\u003e1:2 90-104%; 1:4 85-95%; 1:8 85-95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStability (sealed kit)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003e37 °C, 1 month\u003c\/th\u003e\n\u003ctd\u003eAverage 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003e2-8 °C, 6 months\u003c\/th\u003e\n\u003ctd\u003e95-100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003e2-8 °C, 12 months\u003c\/th\u003e\n\u003ctd\u003e85-98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure (Summary)\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eAdd 100 µL standard or sample; incubate 90 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 2 times; add 100 µL biotin-labeled antigen working solution; incubate 60 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 3 times; add 100 µL HRP-Streptavidin (SABC) working solution; incubate 30 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 5 times; add 90 µL TMB substrate; incubate 10-20 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL stop solution and read at 450 nm immediately.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRequired but not supplied: microplate reader (450 nm), 37 °C incubator (CO₂ incubator not recommended), plate washer or multichannel pipette, calibrated pipettes and tips, sterile tubes, absorbent paper, and deionized or distilled water.\u003c\/p\u003e\n\u003ch2\u003eSample Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSerum and plasma should be diluted at least 1:2 with the sample dilution buffer.\u003c\/li\u003e\n\u003cli\u003eMaximum volume per well: serum, plasma or other liquids 50 µL; cell culture supernatant or cell\/tissue lysate 100 µL.\u003c\/li\u003e\n\u003cli\u003eStore samples below 5 days at 2-8 °C, up to 6 months at -20 °C, or up to 2 years at -80 °C.\u003c\/li\u003e\n\u003cli\u003eAvoid hemolyzed or lipemic samples, and avoid NP-40, Triton X-100 or DTT in lysis buffers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eStore the sealed kit at 2-8 °C and do not freeze. After opening, keep unused strips and standard sealed with desiccant: 1 month at 2-8 °C or 12 months at -20 °C. Keep the biotin-labeled antigen and SABC at 2-8 °C protected from light. Do not mix components from different kits or lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Fine Test","offers":[{"title":"96 T","offer_id":67967519457645,"sku":"EU6603-96T","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EU6603.jpg?v=1794414564","url":"https:\/\/www.ebiohippo.com\/products\/anti-barzolvolimab-antibody-elisa-kit-bhe10814181","provider":"BioHippo","version":"1.0","type":"link"}