{"product_id":"anti-beta-actin-actb-antibody-picoband-hrp-bha21002709","title":"Anti-beta-Actin ACTB Antibody Picoband® (HRP)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-beta-Actin ACTB Antibody Picoband® (HRP) is an antibody for ACTB detection raised in Rabbit (Polyclonal, IgG, HRP), with reported reactivity: Chicken,Human,Monkey,Mouse,Rat,Zebrafish. Commonly used in WB workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ACTB (Actin, beta); UniProt: P60709; NCBI Gene: 60\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, IgG, HRP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 42 kDa, calculated 41737 MW\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP).\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G- actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e cellular localization: Cytoplasm, cytoskeleton, tissue context: Widely expressed, with the highest expression in lung, spleen and thymus..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare ACTB levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Cytoplasm, cytoskeleton\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Widely expressed, with the highest expression in lung, spleen and thymus.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSequence similarities:\u003c\/strong\u003e Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e Biochemicals,Chemical Type\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for P60709: https:\/\/www.uniprot.org\/uniprot\/P60709 - NCBI Gene (ID 60): https:\/\/www.ncbi.nlm.nih.gov\/gene\/60 - Ensembl search (ACTB): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ACTB - Human Protein Atlas search (ACTB): https:\/\/www.proteinatlas.org\/search\/ACTB - PubMed search (ACTB): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ACTB --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067721965933,"sku":"A01263-HRP","price":570.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01263-hrp-beta-actin-primary-antibodies-wb-testing-1.jpg?v=1772609687","url":"https:\/\/www.ebiohippo.com\/products\/anti-beta-actin-actb-antibody-picoband-hrp-bha21002709","provider":"BioHippo","version":"1.0","type":"link"}