{"product_id":"anti-c20orf74-ralgapa2-antibody-picoband-bha21005100","title":"Anti-C20orf74\/RALGAPA2 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-C20orf74\/RALGAPA2 Antibody Picoband® is an antibody reagent for detection of \u003cstrong\u003eRALGAPA2\u003c\/strong\u003e (ring finger protein 169). Researchers commonly use anti-RALGAPA2 antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-C20orf74\/RALGAPA2 Antibody Picoband® catalog # A10416-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RALGAPA2 — Protein NDRG3 (ring finger protein 169). Alternative names: E3 ubiquitin-protein ligase RNF169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; RNF169; KIAA1991\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Polyclonal; Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies context:\u003c\/strong\u003e Host: Rabbit, Reactivity: Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Immunogen affinity purified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e E.coli-derived human C20orf74\/RALGAPA2 recombinant protein (Position: R246-Q1556).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e observed 250 kDa, calculated 41409 MW (reported)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProvided application(s):\u003c\/strong\u003e WB, IHC, Flow, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThese attributes help contextualize how the antibody is commonly selected (host\/clonality\/isotype\/label) and how signals are interpreted across sample types and assay formats.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eFunction:\u003c\/strong\u003e Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization:\u003c\/strong\u003e Nucleoplasm.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTissue details:\u003c\/strong\u003e Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Predicted to enable GTPase activator activity and protein heterodimerization activity. Predicted to be involved in activation of GTPase activity. Predicted to act upstream of or within Ral protein signal transduction; regulation of exocyst localization; and regulation of protein localization. Located in cytosol and plasma membrane.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eQuantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.\u003c\/li\u003e \u003cli\u003eIsoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.\u003c\/li\u003e \u003cli\u003eContext-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e compare relative target abundance and apparent size shifts (e.g., isoforms\/PTMs) across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e assess distribution across tissue compartments and compare staining patterns between groups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and compare shifts after stimulation or differentiation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAcross these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eApparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.\u003c\/li\u003e \u003cli\u003eSpecies reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.\u003c\/li\u003e \u003cli\u003eControl concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO\/KD, peptide competition, or independent assays) to support interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eFor antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProt entry for RALGAPA2 (Q2PPJ7) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/Q2PPJ7 - Ensembl gene summary for RALGAPA2 — Ensembl — https:\/\/www.ensembl.org - HGNC gene symbol report for RALGAPA2 — HGNC — https:\/\/www.genenames.org - Antibody validation concepts (general) — NIH\/NCBI resources — https:\/\/www.ncbi.nlm.nih.gov --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53068621021549,"sku":"A10416-1","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53069057163629,"sku":"A10416-1-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53069057196397,"sku":"A10416-1-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53069057229165,"sku":"A10416-1-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53069057261933,"sku":"A10416-1-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53069057294701,"sku":"A10416-1-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53069057327469,"sku":"A10416-1-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53069057360237,"sku":"A10416-1-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53069057393005,"sku":"A10416-1-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53069057425773,"sku":"A10416-1-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53069057458541,"sku":"A10416-1-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53069057491309,"sku":"A10416-1-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a10416-1-ralgapa2-primary-antibodies-wb-testing-1.jpg?v=1772611512","url":"https:\/\/www.ebiohippo.com\/products\/anti-c20orf74-ralgapa2-antibody-picoband-bha21005100","provider":"BioHippo","version":"1.0","type":"link"}