{"product_id":"anti-ca12-polyclonal-antibody-bha21402003","title":"Anti-CA12 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-CA12 Polyclonal Antibody targets \u003cstrong\u003eCA12\u003c\/strong\u003e (Carbonic anhydrase XII). Commonly used in ELISA, IHC, WB workflows. Research contexts include Oncology \u0026amp; Angiogenesis.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Carbonic anhydrase XII, CA12, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eCA12 (carbonic anhydrase XII) is a transmembrane zinc metalloenzyme that catalyzes reversible hydration of CO₂ to bicarbonate and protons, contributing to extracellular pH regulation. CA12 is expressed in multiple epithelial contexts and is frequently investigated in tumor biology where pH control supports growth and microenvironment adaptation. Common names in the literature include: Carbonic anhydrase XII, CA12, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12. Typical localization reported for this class of target is plasma membrane and\/or cell surface.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a polyclonal reagent, this antibody can provide enhanced sensitivity through multi-epitope recognition. Use it to profile target expression and localization and to compare signal across conditions with matched controls.\u003c\/p\u003e\n\n\u003ch3\u003eAntigen \/ immunogen context\u003c\/h3\u003e\u003cp\u003eE. coli - derived recombinant Human CA12 (Ala25-Leu302).\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, include fresh protease inhibitors and confirm the dominant band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include isotype\/secondary-only controls to assess background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize coating\/capture conditions and validate dynamic range with appropriate standards or reference samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036810568045,"sku":"HT493014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037393772909,"sku":"HT493014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HT493014-abinscience-WB-1.jpg?v=1771744239","url":"https:\/\/www.ebiohippo.com\/products\/anti-ca12-polyclonal-antibody-bha21402003","provider":"BioHippo","version":"1.0","type":"link"}