{"product_id":"anti-cd18-itgb2-antibody-picoband-monoclonal-1a3-2a10-bha21001748","title":"Anti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3\/2A10)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003eITGB2 (Integrin beta-2)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3\/2A10) catalog # M00458-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Mouse Monoclonal Mouse IgG1\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eClone number:\u003c\/strong\u003e Clone: 1A3\/2A10\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18. (reported region: Q404-S769).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 85 kDa; calculated MW: nan\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e IHC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eIntegrin beta-2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit). ITGB2 (INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways. Functional note: Integrin alpha-L\/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M\/beta-2 and alpha-X\/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X\/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M\/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M\/beta-2 is also a receptor for factor X. Integrin alpha- D\/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Triggers neutrophil transmigration during lung injury through PTK2B\/PYK2-mediated activation (PubMed:18587400). Integrin alpha-L\/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). Reported localization: Membrane; Single-pass type I membrane protein. Expression\/tissue context: Leukocytes.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCell Adhesion: Researchers commonly examine how ITGB2 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCell Type Markers: Researchers commonly examine how ITGB2 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCytoskeleton\/ECM: Researchers commonly examine how ITGB2 (Integrin beta-2) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative ITGB2 (Integrin beta-2) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of ITGB2 (Integrin beta-2) across tissue regions and cell types using matched controls.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for P05107: https:\/\/www.uniprot.org\/uniprotkb\/P05107\/entry - PubMed search (ITGB2): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ITGB2 - NCBI Gene search (ITGB2): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ITGB2 - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066872062317,"sku":"M00458-1","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067522900333,"sku":"M00458-1-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067522933101,"sku":"M00458-1-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067522965869,"sku":"M00458-1-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067522998637,"sku":"M00458-1-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067523031405,"sku":"M00458-1-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067523064173,"sku":"M00458-1-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067523096941,"sku":"M00458-1-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067523129709,"sku":"M00458-1-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067523162477,"sku":"M00458-1-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067523195245,"sku":"M00458-1-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067523228013,"sku":"M00458-1-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/M00458-1-ITGB2-primary-antibodies-IHC-testing-1_1.jpg?v=1772608383","url":"https:\/\/www.ebiohippo.com\/products\/anti-cd18-itgb2-antibody-picoband-monoclonal-1a3-2a10-bha21001748","provider":"BioHippo","version":"1.0","type":"link"}