{"product_id":"anti-cd2-cd2-antibody-picoband-bha21007023","title":"Anti-CD2\/CD2 Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-CD2\/CD2 Antibody Picoband® is an antibody targeting \u003cstrong\u003eCD2\u003c\/strong\u003e. Common applications include \u003cstrong\u003eWB, IHC, Flow Cytometry, ELISA\u003c\/strong\u003e. Key specifications include host: \u003cstrong\u003eRabbit\u003c\/strong\u003e; clonality: \u003cstrong\u003ePolyclonal\u003c\/strong\u003e; isotype: \u003cstrong\u003eRabbit IgG\u003c\/strong\u003e; reactivity: \u003cstrong\u003eMouse,Rat\u003c\/strong\u003e; observed MW: \u003cstrong\u003e65 kDa\u003c\/strong\u003e; calculated MW: \u003cstrong\u003e38415 MW\u003c\/strong\u003e.\u003c\/p\u003e\u003cp\u003eBoster Bio Anti-CD2\/CD2 Antibody catalog # PA1743. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD2 — T-cell surface antigen CD2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Mouse,Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight guidance:\u003c\/strong\u003e Observed: 65 kDa; Calculated: 38415 MW\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eSpecificity note:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eProtein function (datasheet):\u003c\/strong\u003e CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48\/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eScientific background (datasheet):\u003c\/strong\u003e CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11\/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies ed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al. (1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3\/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCellular localization (datasheet):\u003c\/strong\u003e Membrane; Single-pass type I membrane protein.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSequence similarities (datasheet):\u003c\/strong\u003e Contains 1 Ig-like C2-type (immunoglobulin-like) domain.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCommonly studied in contexts related to Adaptive Immunity,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells,T Cells,T Lymphocytic Lineage.\u003c\/li\u003e\n\u003cli\u003eSupports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003eUseful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Compare relative target abundance and apparent size\/isoforms across samples; interpret bands in light of expected MW and potential PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify target-positive populations in single-cell suspensions; pair with viability and isotype\/FMO controls conceptually.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity (datasheet):\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., KO\/KD, blocking peptide, or isotype controls) to support specificity interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAs a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.\u003c\/p\u003e\u003c!-- Sources (internal):\u003cbr\u003e- Product datasheet fields provided in this catalog row (vendor-supplied).\u003cbr\u003e- UniProt Knowledgebase (general protein annotations): https:\/\/www.uniprot.org\/\u003cbr\u003e- NCBI Gene (general gene-level references): https:\/\/www.ncbi.nlm.nih.gov\/gene\/\u003cbr\u003e--\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53069796245869,"sku":"PA1743","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53070205190509,"sku":"PA1743-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53070205223277,"sku":"PA1743-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53070205256045,"sku":"PA1743-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53070205288813,"sku":"PA1743-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53070205321581,"sku":"PA1743-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53070205354349,"sku":"PA1743-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53070205387117,"sku":"PA1743-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53070205419885,"sku":"PA1743-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53070205452653,"sku":"PA1743-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53070205485421,"sku":"PA1743-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53070205518189,"sku":"PA1743-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pa1743-1-WB-anti-cd2-antibody.jpg?v=1772558230","url":"https:\/\/www.ebiohippo.com\/products\/anti-cd2-cd2-antibody-picoband-bha21007023","provider":"BioHippo","version":"1.0","type":"link"}