{"product_id":"anti-cd20-igg-antibody-biotin-labeled-bha18200068","title":"Anti-CD20 IgG Antibody, Biotin-labeled","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cp class=\"bhp-cat-pill\"\u003eImmunotherapy\u003c\/p\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eBiotinylated anti-CD20 IgG antibody is a purified recombinant antibody which recognizes human CD20 antigen. This antibody has been tested for specific binding to purified human CD20 protein.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eIgG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eBiotin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eProtein A affinity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e≥90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eHeavy Chain: 53 kDa; Light Chain 23 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003e8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAt least 12 months at -80°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eValidated Applications\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eWestern Blot\u003c\/li\u003e\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAssay conditions:\u003c\/strong\u003e Functional validation: The antibody was validated by ELISA using a purified CD20 antigen. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl\/well at a concentration of 4 µg\/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl\/well of purified biotinylated anti-CD20 antibody (BPS Bioscience #101207), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.\u003c\/p\u003e\n\u003ch2\u003eImmunogen \/ Antigen Information\u003c\/h2\u003e\n\u003cp\u003eImmunogen information not provided. Please refer to the product datasheet for detailed antigen information.\u003c\/p\u003e\n\u003ch2\u003eCross-Reactivity \u0026amp; Specificity\u003c\/h2\u003e\n\u003cp\u003eCross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eBiosafety Level:\u003c\/strong\u003e Not applicable (BSL-1)\u003c\/div\u003e\n\u003cp\u003eAvoid freeze\/thaw cycles.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e71209\u003c\/li\u003e\n\u003cli\u003e100836\u003c\/li\u003e\n\u003cli\u003e100860\u003c\/li\u003e\n\u003cli\u003e78606\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"50 ug","offer_id":53327927509357,"sku":"101207-1","price":335.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53327933604205,"sku":"101207-2","price":460.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/101207_gel.png?v=1778728312","url":"https:\/\/www.ebiohippo.com\/products\/anti-cd20-igg-antibody-biotin-labeled-bha18200068","provider":"BioHippo","version":"1.0","type":"link"}