{"product_id":"anti-cd74-antibody-bha21002811","title":"Anti-CD74 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAnti-CD74 Antibody is an antibody for CD74 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD74 (glutamate metabotropic receptor 5); UniProt: P04233\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit, Polyclonal, Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 178 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, IHC, IF, ICC, Flow Cytometry, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eVendor description (summary):\u003c\/strong\u003e Boster Bio Anti-CD74 Antibody Picoband® catalog # A01340-2.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBiological context:\u003c\/strong\u003e G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpression and localization notes:\u003c\/strong\u003e tissue context: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells..\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e Compare CD74 levels across samples and conditions using appropriate loading and biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry (IHC):\u003c\/strong\u003e Evaluate spatial distribution of CD74 in tissue sections, considering fixation and antigen retrieval effects.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence \/ ICC:\u003c\/strong\u003e Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e Quantify CD74-positive populations in single-cell suspensions with appropriate gating and controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAccount for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e \u003cli\u003eUse positive\/negative biological controls where possible (e.g., known-expressing cells\/tissues, knockdown\/knockout models) and include appropriate secondary-only\/isotype controls for imaging workflows.\u003c\/li\u003e \u003c\/ul\u003e\u003ch2\u003eAdditional product notes (from provided fields)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground:\u003c\/strong\u003e HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details:\u003c\/strong\u003e Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResearch category:\u003c\/strong\u003e Subcellular Markers,Tags \u0026amp; Cell Markers,Vesicle Transport\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt entry for P04233: https:\/\/www.uniprot.org\/uniprot\/P04233 - Ensembl search (CD74): https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD74 - Human Protein Atlas search (CD74): https:\/\/www.proteinatlas.org\/search\/CD74 - PubMed search (CD74): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD74 --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53067726586221,"sku":"A01340-2","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53068031361389,"sku":"A01340-2-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53068031394157,"sku":"A01340-2-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53068031426925,"sku":"A01340-2-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53068031459693,"sku":"A01340-2-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53068031492461,"sku":"A01340-2-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53068031525229,"sku":"A01340-2-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53068031557997,"sku":"A01340-2-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53068031590765,"sku":"A01340-2-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53068031623533,"sku":"A01340-2-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53068031656301,"sku":"A01340-2-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53068031689069,"sku":"A01340-2-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01340-2-cd74-primary-antibodies-ihc-testing-1.jpg?v=1772609709","url":"https:\/\/www.ebiohippo.com\/products\/anti-cd74-antibody-bha21002811","provider":"BioHippo","version":"1.0","type":"link"}