{"product_id":"anti-cdk1-polyclonal-antibody-bha21404656","title":"Anti-CDK1 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against CDK1 (Cell division control protein 2 homolog) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eCDK1 encodes a cyclin-dependent kinase (CDK) family serine\/threonine kinase involved in phosphorylation-driven signaling. CDKs coordinate cell-state transitions by partnering with regulatory proteins and substrates such as RB-family proteins and transcriptional regulators. Antibodies against CDK1 are commonly used to study kinase expression, activation-state changes (often via upstream cues), and pathway rewiring in proliferation and cancer models.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eCDK family kinases are typically detected in nuclear and cytoplasmic compartments; distribution can shift with cell-cycle state and binding partners.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eCell Signaling\u003c\/em\u003e research. Application suitability (e.g., ELISA, IF, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Mouse, Rat).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of CDK1, and a positive control sample known to express CDK1.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eGenerated using an immunogen derived from E. coli - derived recombinant Human CDK1 (Met1-Met297).. When interpreting results, note that epitope accessibility can vary with denaturation, fixation, or post‑translational modifications; empirical optimization is recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036902023533,"sku":"HY308014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037486539117,"sku":"HY308014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HY308014-abinscience-WB-1.jpg?v=1771745471","url":"https:\/\/www.ebiohippo.com\/products\/anti-cdk1-polyclonal-antibody-bha21404656","provider":"BioHippo","version":"1.0","type":"link"}