{"product_id":"anti-cx3cr1-extracellular-apc-antibody-bha21300269","title":"Anti-CX3CR1 (extracellular)-APC Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-CX3CR1 (extracellular)-APC Antibody is an antibody targeting Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1 Polyclonal raised in Rabbit (Allophycocyanin (APC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1 (also reported as Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Extracellular, N-terminus..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Rat - identical Will not recognize human CX3CR1 (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis (unlabeled antibody, #ACR-058), and direct flow cytometry (labeled antibody)..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Allophycocyanin (APC) (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eCX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eProfiling immune-marker expression across cell subsets with single-cell or flow-based readouts.\u003c\/li\u003e   \u003cli\u003eConnecting receptor\/ligand levels to activation state and cytokine programs.\u003c\/li\u003e   \u003cli\u003eApplying genetic perturbation or orthogonal assays to support specificity and interpretation.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eFlow cytometry (direct\/indirect): quantify target-positive populations and shifts in expression across subsets.\u003c\/li\u003e   \u003cli\u003eLive cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: RIC-001-APC.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-CR058; Negative control: RIC-001-APC.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"50 mcl \/ 1","offer_id":53064824389997,"sku":"ACR-058-APC-50MCL-1","price":797.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 2","offer_id":53064884158829,"sku":"ACR-058-APC-50MCL-2","price":1397.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 5","offer_id":53064884191597,"sku":"ACR-058-APC-50MCL-5","price":2743.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 10","offer_id":53064884224365,"sku":"ACR-058-APC-50MCL-10","price":3764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ACR-058-APC_BV2_2.5mic_FC1.png?v=1772460953","url":"https:\/\/www.ebiohippo.com\/products\/anti-cx3cr1-extracellular-apc-antibody-bha21300269","provider":"BioHippo","version":"1.0","type":"link"}