{"product_id":"anti-cyclophilin-a-ppia-antibody-picoband-bha21001169","title":"Anti-Cyclophilin A\/PPIA Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003ePPIA (Peptidyl-prolyl cis-trans isomerase A)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-Cyclophilin A\/PPIA Antibody Picoband® catalog # A01308. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E.coli-derived human Cyclophilin A recombinant protein (Position: T116-E165). Human Cyclophilin A shares 98% and 95.9% amino acid (aa) sequence identity with mouse and rat Cyclophilin A, respectively. (reported region: T116-E165).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 185 kDa; calculated MW: 18012 MW\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human,Mouse,Rat\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e Flow Cytometry, IF, IHC, IHC-F, ICC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003ePeptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A. Cyclophilin A (PPIA), Peptidylprolyl isomerase A, is an enzyme that in humans is encoded by the PPIA gene. Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Cyclophilin A is also a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis\/trans isomerase activity and are believed to be involved in protein folding and\/or intracellular protein transport. And Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1 (HIV-1). Additionally, Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication. Functional note: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Reported localization: Cytoplasm . Secreted . Secretion occurs in response to oxidative stress in vascular smooth muscle through a vesicular secretory pathway that involves actin remodeling and myosin II activation, and mediates ERK1\/2 activation. Expression\/tissue context: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMechanistic pathway studies: Researchers commonly examine how PPIA (Peptidyl-prolyl cis-trans isomerase A) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eBiomarker profiling across models: Researchers commonly examine how PPIA (Peptidyl-prolyl cis-trans isomerase A) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003ePerturbation-response experiments (time-course\/dose–response): Researchers commonly examine how PPIA (Peptidyl-prolyl cis-trans isomerase A) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative PPIA (Peptidyl-prolyl cis-trans isomerase A) levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of PPIA (Peptidyl-prolyl cis-trans isomerase A) across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eIF\/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation\/permeabilization and epitope accessibility.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for P62937: https:\/\/www.uniprot.org\/uniprotkb\/P62937\/entry - PubMed search (PPIA): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PPIA - NCBI Gene search (PPIA): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PPIA - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066851975533,"sku":"A01308","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067311907181,"sku":"A01308-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067311939949,"sku":"A01308-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067311972717,"sku":"A01308-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067312005485,"sku":"A01308-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067312038253,"sku":"A01308-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067312071021,"sku":"A01308-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067312103789,"sku":"A01308-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067312136557,"sku":"A01308-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067312169325,"sku":"A01308-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067312202093,"sku":"A01308-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067312234861,"sku":"A01308-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01308-cyclophilin-a-primary-antibodies-wb-testing-1.jpg?v=1772608279","url":"https:\/\/www.ebiohippo.com\/products\/anti-cyclophilin-a-ppia-antibody-picoband-bha21001169","provider":"BioHippo","version":"1.0","type":"link"}