{"product_id":"anti-dsred-nanobody-saa1152-bha21409472","title":"Anti-dsRed Nanobody (SAA1152)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-dsRed Nanobody (SAA1152) is intended for detection of \u003cstrong\u003edsRed Nanobody\u003c\/strong\u003e. Commonly used in ELISA, IF applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Detect target localization by IF\/ICC in cultured cells, Develop ELISA pairing using capture\/detection antibodies and a standard curve, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Fluorescent protein raspberry.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Alpaca; Clonality: Monoclonal; Isotype: VHH-8His-Cys-tag; Purification: Purified by Nickel column.; Format: Liquid; Reactivity: Discosoma sp. LW-2004.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003edsRed Nanobody (Fluorescent protein raspberry) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular \u0026amp; Cellular Biology. Based on the annotated name\/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037061570925,"sku":"JN056013-50UG","price":158.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037791478125,"sku":"JN056013-100UG","price":258.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/JN056013-SDS-PAGE-1.jpg?v=1771748295","url":"https:\/\/www.ebiohippo.com\/products\/anti-dsred-nanobody-saa1152-bha21409472","provider":"BioHippo","version":"1.0","type":"link"}