{"product_id":"anti-dykddddk-flag-affinity-gel-bhd20800009","title":"Anti-DYKDDDDK (Flag) Affinity Gel","description":"\u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eAnti-Flag affinity purification gel (secretory protein or Membrane Protein) is prepared by covalent coupling of high-quality mouse IgG2b monoclonal antibody (clone number: 3F5) and 4% highly cross-linked agarose gel. This product has a high loading capacity of Flag-tagged fusion protein (at least 1.1 mg protein\/mL gel) and less non-specific binding of impurities. It can be used for purification and immunoprecipitation (IP) of fusion proteins (secretory protein or membrane protein) with Flag tags.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eAnti-Flag affinity purification gel (secretory protein or Membrane Protein) can bind to Met-modified N-terminal Flag fusion protein (Met-FLAG–Protein), N-terminal Flag fusion protein (FLAG–Protein), and C-terminal Flag fusion protein (Protein-FLAG).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFeature\u003c\/span\u003e\u003c\/b\u003es\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eHigh specificity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e High-quality mouse IgG2b monoclonal antibody is used, which can specifically adsorb the Flag protein sequence.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003eHigh loading capacity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Loading capacity \u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e≥ 1.1 mg protein\/mL gel.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003eHigh purity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Less non-specific binding of impurities, resulting in high-purity Flag-tagged protein.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003eWide range of uses:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Can be used for protein purification or IP.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003eMultiple types of binding proteins:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Can bind to Met-modified N-terminal Flag fusion protein (Met-Flag–Protein), N-terminal Flag fusion protein (Flag–Protein), C-terminal Flag fusion protein (Protein-Flag)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"19\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"31.1600%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eClone\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"68.8200%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003e3F5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"31.1600%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eIsotype\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"68.8200%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eIgG2b\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"31.1600%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"68.8200%\"\u003e\n\u003cp\u003ePurification of secreted or membrane proteins, immunoprecipitation（IP）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"31.1600%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eProtein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"68.8200%\"\u003e\n\u003cp\u003e≥1.1 mg protein\/mL gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"31.1600%\"\u003e\n\u003cp class=\"16\"\u003e\u003cspan\u003eBuffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"68.8200%\"\u003e\n\u003cp\u003eTBS ，50%glycerin ，pH7.4 ，contains 0.02% (w\/v) sodium azide\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003eThis product should be stored at 2~8℃ for 1 year. Cryopreservation in solutions without glycerol is prohibited!\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003ePurification of secreted or membrane proteins, immunoprecipitation（IP\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eHigh specificity and efficiently\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20585-1_5eca4d85-5741-464b-aa56-cda2c58a337c.png?v=1750301108\"\u003e \u003c\/span\u003e\u003c\/b\u003eFigure 1. Exhibits high specificity and efficiently binds Flag tags at any position, even at very low target protein expression levels using Anti-DYKDDDDK (Flag) Affinity Gel\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eImproved target protein yield and purity\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20585-2_eb28f107-07c8-4ffc-917d-c08fbe74c98f.png?v=1750301108\"\u003e \u003c\/span\u003e\u003c\/b\u003eFigure 2. Anti-DYKDDDDK (Flag) Affinity Gel has higher yield and purity of secretory or membrane proteins compared to other suppliers.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003ePublication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli class=\"15\"\u003e\u003ca href=\"https:\/\/www.cell.com\/immunity\/abstract\/S1074-7613(24)00142-0?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1074761324001420%3Fshowall%3Dtrue\"\u003e\u003cspan\u003eXiao J, Wang S, Chen L, et al. 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages. Immunity. 2024;57(5):1087-1104.e7. doi:10.1016\/j.immuni.2024.03.021(IF:32.4)\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\n\u003ca href=\"https:\/\/molecular-cancer.biomedcentral.com\/articles\/10.1186\/s12943-023-01912-w\"\u003e\u003cspan\u003eZheng Z, Zeng X, Zhu Y, et al. CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p\/CYP1B1 axis. Mol Cancer. 2024;23(1):4. Published 2024 Jan 6. doi:10.1186\/s12943-023-01912-w(IF:37.3)\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eLin J, Jiang X, Dong M, et al. Hepatokine Pregnancy Zone Protein Governs the Diet-Induced Thermogenesis Through Activating Brown Adipose Tissue. Adv Sci (Weinh). 2021;8(21):e2101991. doi:10.1002\/advs.202101991(IF:16.806)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eHan L, Zhang F, Liu Y, et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med. 2022;12(6):e850. doi:10.1002\/ctm2.850(IF:11.492)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eChen X, Duan Y, Qiao F, et al. A secreted fungal effector suppresses rice immunity through host histone hypoacetylation [published online ahead of print, 2022 May 20]. New Phytol. 2022;10.1111\/nph.18265. doi:10.1111\/nph.18265(IF:10.152)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eChen Y, Yang L, Lu Y, et al. Up-regulation of NMRK2 mediated by TFE3 fusions is the key for energy metabolism adaption of Xp11.2 translocation renal cell carcinoma. Cancer Lett. 2022;538:215689. doi:10.1016\/j.canlet.2022.215689(IF:8.679)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eChen X, Li X, Li P, et al. Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence. J Integr Plant Biol. 2021;63(2):409-425. doi:10.1111\/jipb.13066(IF:7.061)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eTang C, Zhou Y, Sun W, et al. Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway. Cancers (Basel). 2022;14(9):2338. Published 2022 May 9. doi:10.3390\/cancers14092338(IF:6.639)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eZheng G, Jiang C, Li Y, et al. TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia\/cardiomyopathy. Protein Cell. 2019;10(2):104-119. doi:10.1007\/s13238-018-0563-2(IF:6.228)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"15\"\u003e\u003cspan\u003eChen X, Tang J, Pei Z, et al. The 'pears and lemons' protein UvPal1 regulates development and virulence of Ustilaginoidea virens. Environ Microbiol. 2020;22(12):5414-5432. doi:10.1111\/1462-2920.15284(IF:4.933)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: If the protein is to remain active, which substances should be used for elution?\u003c\/p\u003e\n\u003cp\u003eA: Non-denaturing elution method can be used.\u003c\/p\u003e\n\u003cp\u003eQ: How does this product compare with those from the imported manufacturers?\u003c\/p\u003e\n\u003cp\u003eA: The protein binding capacity of Yeasen 20585 is nearly twice that of the imported S* brand.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/643ab9c00bbccb209e95bddd9c550ec3_1024x1024.png?v=1769053377\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eQ: We used the flag peptide for elution, but it couldn't be completely removed?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: The loading capacity of the medium is already relatively low, and the elution concentration might be quite low. There is a possibility that due to the low concentration, no distinct bands will be visible. It is recommended to increase the elution intensity in the next step.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20585ES--MSDS-HB250806.pdf?v=1754445669\"\u003e20585_MSDS_HB250806_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20585ES-Manual-Ver.EN20250806.pdf?v=1754445685\"\u003e20585_Manual_Ver.EN20250806.pdf\u003c\/a\u003e\u003cimg\u003e\u003c\/p\u003e\n\u003ch3 class=\"4ever-article\"\u003e\u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003eRelated Blog:\u003c\/span\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/molecular-biology\/anti-flag-affinity-gel-a-reliable-partner-for-ip-and-protein-purification?_pos=1\u0026amp;_psq=Flag\u0026amp;_ss=e\u0026amp;_v=1.0\"\u003eAnti-Flag Affinity Gel: A Reliable Partner for IP and Protein Purification\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/protein\/yeasen-anti-dykddddk-flag-affinity-gel-precision-purification-for-low-abundance-membrane-proteins?_pos=2\u0026amp;_psq=Flag\u0026amp;_ss=e\u0026amp;_v=1.0\"\u003eYeasen Anti-DYKDDDDK (Flag) Affinity Gel: Precision Purification for Low-Abundance Membrane Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/protein\/the-tool-for-capturing-flag-ha-myc-tagged-proteins\"\u003eThe Tool for Capturing Flag\/HA\/Myc-Tagged Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong data-end=\"430\" data-start=\"410\"\u003eTry it for free!\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eGet a \u003cstrong data-end=\"454\" data-start=\"439\"\u003efree sample\u003c\/strong\u003e of Anti-Flag and validate its performance in your own lab.\u003cbr data-end=\"527\" data-start=\"524\"\u003e👉 [\u003ca href=\"https:\/\/share.hsforms.com\/2Q3Kq7O7FSeSriECdgkdCpQsivym\" rel=\"noopener\" target=\"_blank\"\u003eRequest Sample Now\u003c\/a\u003e\u003ca rel=\"noopener\" href=\"https:\/\/sivym.share.hsforms.com\/2tJ9_i9tQRu2eEEseCHkxGQ\" target=\"_blank\"\u003e\u003c\/a\u003e]. \u003cstrong\u003eShipping Worldwide!\u003c\/strong\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1 mL","offer_id":53286019826029,"sku":"20585ES03","price":385.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20585-1_5eca4d85-5741-464b-aa56-cda2c58a337c.png?v=1777929288","url":"https:\/\/www.ebiohippo.com\/products\/anti-dykddddk-flag-affinity-gel-bhd20800009","provider":"BioHippo","version":"1.0","type":"link"}