{"product_id":"anti-elafin-skalp-pi3-antibody-monoclonal-c7-bha21001758","title":"Anti-Elafin\/Skalp PI3 Antibody  (monoclonal, C7)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003ePI3 (Glial fibrillary acidic protein)\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-Elafin\/Skalp PI3 Antibody Picoband® (monoclonal, C7) catalog # M00364. Tested in ELISA, IHC applications. This antibody reacts with Human.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Mouse Monoclonal Mouse IgG1\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eClone number:\u003c\/strong\u003e Clone: C7\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E. coli-derived human Elafin\/Skalp recombinant protein (Position: A61-Q117). (reported region: A61-Q117).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 25 kDa; calculated MW: nan\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e ELISA, IHC\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eGlial fibrillary acidic protein; peptidase inhibitor 3. Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. Functional note: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Reported localization: Secreted. Expression\/tissue context: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCell Cycle: Researchers commonly examine how PI3 (Glial fibrillary acidic protein) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eImmunology: Researchers commonly examine how PI3 (Glial fibrillary acidic protein) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eInnate Immunity: Researchers commonly examine how PI3 (Glial fibrillary acidic protein) relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eIHC\/IHC-F: assess spatial distribution of PI3 (Glial fibrillary acidic protein) across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for P19957: https:\/\/www.uniprot.org\/uniprotkb\/P19957\/entry - PubMed search (PI3): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PI3 - NCBI Gene search (PI3): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PI3 - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066872226157,"sku":"M00364","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067526865261,"sku":"M00364-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067526898029,"sku":"M00364-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067526930797,"sku":"M00364-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067526963565,"sku":"M00364-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067526996333,"sku":"M00364-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067527029101,"sku":"M00364-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067527061869,"sku":"M00364-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067527094637,"sku":"M00364-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067527127405,"sku":"M00364-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067527160173,"sku":"M00364-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067527192941,"sku":"M00364-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/M00364-Elafin-primary-antibodies-IHC-testing-1.jpg?v=1772608404","url":"https:\/\/www.ebiohippo.com\/products\/anti-elafin-skalp-pi3-antibody-monoclonal-c7-bha21001758","provider":"BioHippo","version":"1.0","type":"link"}